Although the development of super-resolution microscopy methods dates back to 1994, relevant applications in plant cell imaging only started to emerge in 2010. Since then, the principal super-resolution methods, including structured-illumination microscopy (SIM), photoactivation localization microscopy (PALM), stochastic optical reconstruction microscopy (STORM), and stimulated emission depletion microscopy (STED), have been implemented in plant cell research. However, progress has been limited due to the challenging properties of plant material. Here we summarize the basic principles of existing super-resolution methods and provide examples of applications in plant science. The limitations imposed by the nature of plant material are reviewed and the potential for future applications in plant cell imaging is highlighted.

Centre of the Region Haná for Biotechnological and Agricultural Research Palacký University Olomouc Faculty of Science Šlechtitelů 27 783 71 Olomouc Czech Republic

References provided by Crossref.org

Correlative Light-Environmental Scanning Electron Microscopy of Plasma Membrane Efflux Carriers of Plant Hormone Auxin

Comparing Super-Resolution Microscopy Techniques to Analyze Chromosomes

Prospects and limitations of expansion microscopy in chromatin ultrastructure determination

Cytokinin fluoroprobe reveals multiple sites of cytokinin perception at plasma membrane and endoplasmic reticulum

Super-Resolution Microscopy Reveals Diversity of Plant Centromere Architecture

Multicolour three dimensional structured illumination microscopy of immunolabeled plant microtubules and associated proteins