The impact of silica encapsulated cobalt zinc ferrite nanoparticles on DNA, lipids and proteins of rat bone marrow mesenchymal stem cells
Jazyk angličtina Země Anglie, Velká Británie Médium print-electronic
Typ dokumentu časopisecké články
- Klíčová slova
- Comet assay, cytotoxicity, genotoxicity, lipid peroxidation, protein oxidative damage,
- MeSH
- barvení a značení MeSH
- buněčné kultury MeSH
- dinoprost analogy a deriváty MeSH
- isoprostany metabolismus MeSH
- karbonylace proteinů účinky léků MeSH
- kobalt chemie toxicita MeSH
- kontrastní látky chemie toxicita MeSH
- krysa rodu Rattus MeSH
- kultivované buňky MeSH
- magnetická rezonanční tomografie MeSH
- metabolismus lipidů účinky léků MeSH
- mezenchymální kmenové buňky účinky léků metabolismus ultrastruktura MeSH
- nanočástice chemie toxicita MeSH
- oxid křemičitý chemie toxicita MeSH
- poškození DNA * MeSH
- povrchové vlastnosti MeSH
- proliferace buněk účinky léků MeSH
- sloučeniny zinku chemie toxicita MeSH
- transmisní elektronová mikroskopie MeSH
- viabilita buněk účinky léků MeSH
- vztah mezi dávkou a účinkem léčiva MeSH
- železité sloučeniny chemie toxicita MeSH
- zvířata MeSH
- Check Tag
- krysa rodu Rattus MeSH
- zvířata MeSH
- Publikační typ
- časopisecké články MeSH
- Názvy látek
- 8-epi-prostaglandin F2alpha MeSH Prohlížeč
- dinoprost MeSH
- isoprostany MeSH
- kobalt MeSH
- kontrastní látky MeSH
- oxid křemičitý MeSH
- sloučeniny zinku MeSH
- železité sloučeniny MeSH
Nanomaterials are currently the subject of intense research due to their wide variety of potential applications in the biomedical, optical and electronic fields. We prepared and tested cobalt zinc ferrite nanoparticles (Co0.5Zn0.5Fe2O4+γ [CZF-NPs]) encapsulated by amorphous silica in order to find a safe contrast agent and magnetic label for tracking transplanted cells within an organism using magnetic resonance imaging (MRI). Rat mesenchymal stem cells (rMSCs) were labeled for 48 h with a low, medium or high dose of CZF-NPs (0.05; 0.11 or 0.55 mM); silica NPs (Si-NPs; 0.11 mM) served as a positive control. The internalization of NPs into cells was verified by transmission electron microscopy. Biological effects were analyzed at the end of exposure and after an additional 72 h of cell growth without NPs. Compared to untreated cells, Annexin V/Propidium Iodide labeling revealed no significant cytotoxicity for any group of treated cells and only a high dose of CZF-NPs slowed down cell proliferation and induced DNA damage, manifested as a significant increase of DNA-strand breaks and oxidized DNA bases. This was accompanied by high concentrations of 15-F2t-isoprostane and carbonyl groups, demonstrating oxidative injury to lipids and proteins, respectively. No harmful effects were detected in cells exposed to the low dose of CZF-NPs. Nevertheless, the labeled cells still exhibited an adequate relaxation rate for MRI in repeated experiments and ICP-MS confirmed sufficient magnetic label concentrations inside the cells. The results suggest that the silica-coated CZF-NPs, when applied at a non-toxic dose, represent a promising contrast agent for cell labeling.
b Institute of Physics AS CR v v i Prague Czech Republic
c Institute for Clinical and Experimental Medicine Prague Czech Republic
e University of Chemistry and Technology Prague Czech Republic and
f New Technologies Research Centre University of West Bohemia Pilsen Czech Republic
Citace poskytuje Crossref.org
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