The impact of silica encapsulated cobalt zinc ferrite nanoparticles on DNA, lipids and proteins of rat bone marrow mesenchymal stem cells
Language English Country England, Great Britain Media print-electronic
Document type Journal Article
- Keywords
- Comet assay, cytotoxicity, genotoxicity, lipid peroxidation, protein oxidative damage,
- MeSH
- Staining and Labeling MeSH
- Cell Culture Techniques MeSH
- Dinoprost analogs & derivatives MeSH
- Isoprostanes metabolism MeSH
- Protein Carbonylation drug effects MeSH
- Cobalt chemistry toxicity MeSH
- Contrast Media chemistry toxicity MeSH
- Rats MeSH
- Cells, Cultured MeSH
- Magnetic Resonance Imaging MeSH
- Lipid Metabolism drug effects MeSH
- Mesenchymal Stem Cells drug effects metabolism ultrastructure MeSH
- Nanoparticles chemistry toxicity MeSH
- Silicon Dioxide chemistry toxicity MeSH
- DNA Damage * MeSH
- Surface Properties MeSH
- Cell Proliferation drug effects MeSH
- Zinc Compounds chemistry toxicity MeSH
- Microscopy, Electron, Transmission MeSH
- Cell Survival drug effects MeSH
- Dose-Response Relationship, Drug MeSH
- Ferric Compounds chemistry toxicity MeSH
- Animals MeSH
- Check Tag
- Rats MeSH
- Animals MeSH
- Publication type
- Journal Article MeSH
- Names of Substances
- 8-epi-prostaglandin F2alpha MeSH Browser
- Dinoprost MeSH
- Isoprostanes MeSH
- Cobalt MeSH
- Contrast Media MeSH
- Silicon Dioxide MeSH
- Zinc Compounds MeSH
- Ferric Compounds MeSH
Nanomaterials are currently the subject of intense research due to their wide variety of potential applications in the biomedical, optical and electronic fields. We prepared and tested cobalt zinc ferrite nanoparticles (Co0.5Zn0.5Fe2O4+γ [CZF-NPs]) encapsulated by amorphous silica in order to find a safe contrast agent and magnetic label for tracking transplanted cells within an organism using magnetic resonance imaging (MRI). Rat mesenchymal stem cells (rMSCs) were labeled for 48 h with a low, medium or high dose of CZF-NPs (0.05; 0.11 or 0.55 mM); silica NPs (Si-NPs; 0.11 mM) served as a positive control. The internalization of NPs into cells was verified by transmission electron microscopy. Biological effects were analyzed at the end of exposure and after an additional 72 h of cell growth without NPs. Compared to untreated cells, Annexin V/Propidium Iodide labeling revealed no significant cytotoxicity for any group of treated cells and only a high dose of CZF-NPs slowed down cell proliferation and induced DNA damage, manifested as a significant increase of DNA-strand breaks and oxidized DNA bases. This was accompanied by high concentrations of 15-F2t-isoprostane and carbonyl groups, demonstrating oxidative injury to lipids and proteins, respectively. No harmful effects were detected in cells exposed to the low dose of CZF-NPs. Nevertheless, the labeled cells still exhibited an adequate relaxation rate for MRI in repeated experiments and ICP-MS confirmed sufficient magnetic label concentrations inside the cells. The results suggest that the silica-coated CZF-NPs, when applied at a non-toxic dose, represent a promising contrast agent for cell labeling.
b Institute of Physics AS CR v v i Prague Czech Republic
c Institute for Clinical and Experimental Medicine Prague Czech Republic
e University of Chemistry and Technology Prague Czech Republic and
f New Technologies Research Centre University of West Bohemia Pilsen Czech Republic
References provided by Crossref.org
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