The effects of different cross-linking conditions on collagen-based nanocomposite scaffolds-an in vitro evaluation using mesenchymal stem cells
Jazyk angličtina Země Anglie, Velká Británie Médium electronic
Typ dokumentu srovnávací studie, hodnotící studie, časopisecké články, práce podpořená grantem
- MeSH
- analýza selhání vybavení MeSH
- biokompatibilní materiály chemická syntéza MeSH
- buněčná adheze fyziologie MeSH
- design vybavení MeSH
- kolagen chemie MeSH
- kostní matrix chemie MeSH
- kostní náhrady chemická syntéza MeSH
- lidé MeSH
- mezenchymální kmenové buňky cytologie fyziologie MeSH
- nanokompozity chemie ultrastruktura MeSH
- proliferace buněk fyziologie MeSH
- reagencia zkříženě vázaná chemie MeSH
- testování materiálů MeSH
- tkáňové podpůrné struktury * MeSH
- transplantace mezenchymálních kmenových buněk přístrojové vybavení metody MeSH
- velikost částic MeSH
- viabilita buněk fyziologie MeSH
- Check Tag
- lidé MeSH
- Publikační typ
- časopisecké články MeSH
- hodnotící studie MeSH
- práce podpořená grantem MeSH
- srovnávací studie MeSH
- Názvy látek
- biokompatibilní materiály MeSH
- kolagen MeSH
- kostní náhrady MeSH
- reagencia zkříženě vázaná MeSH
Nanocomposite scaffolds which aimed to imitate a bone extracellular matrix were prepared for bone surgery applications. The scaffolds consisted of polylactide electrospun nano/sub-micron fibres, a natural collagen matrix supplemented with sodium hyaluronate and natural calcium phosphate nano-particles (bioapatite). The mechanical properties of the scaffolds were improved by means of three different cross-linking agents: N-(3-dimethylamino propyl)-N'-ethylcarbodiimide hydrochloride and N-hydroxysuccinimide in an ethanol solution (EDC/NHS/EtOH), EDC/NHS in a phosphate buffer saline solution (EDC/NHS/PBS) and genipin. The effect of the various cross-linking conditions on the pore size, structure and mechanical properties of the scaffolds were subsequently studied. In addition, the mass loss, the swelling ratio and the pH of the scaffolds were determined following their immersion in a cell culture medium. Furthermore, the metabolic activity of human mesenchymal stem cells (hMSCs) cultivated in scaffold infusions for 2 and 7 days was assessed. Finally, studies were conducted of cell adhesion, proliferation and penetration into the scaffolds. With regard to the structural stability of the tested scaffolds, it was determined that EDC/NHS/PBS and genipin formed the most effectively cross-linked materials. Moreover, it was discovered that the genipin cross-linked scaffold also provided the best conditions for hMSC cultivation. In addition, the infusions from all the scaffolds were found to be non-cytotoxic. Thus, the genipin and EDC/NHS/PBS cross-linked scaffolds can be considered to be promising biomaterials for further in vivo testing and bone surgery applications.
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