The effects of different cross-linking conditions on collagen-based nanocomposite scaffolds-an in vitro evaluation using mesenchymal stem cells

. 2015 Nov 20 ; 10 (6) : 065008. [epub] 20151120

Jazyk angličtina Země Anglie, Velká Británie Médium electronic

Typ dokumentu srovnávací studie, hodnotící studie, časopisecké články, práce podpořená grantem

Perzistentní odkaz   https://www.medvik.cz/link/pmid26586611

Nanocomposite scaffolds which aimed to imitate a bone extracellular matrix were prepared for bone surgery applications. The scaffolds consisted of polylactide electrospun nano/sub-micron fibres, a natural collagen matrix supplemented with sodium hyaluronate and natural calcium phosphate nano-particles (bioapatite). The mechanical properties of the scaffolds were improved by means of three different cross-linking agents: N-(3-dimethylamino propyl)-N'-ethylcarbodiimide hydrochloride and N-hydroxysuccinimide in an ethanol solution (EDC/NHS/EtOH), EDC/NHS in a phosphate buffer saline solution (EDC/NHS/PBS) and genipin. The effect of the various cross-linking conditions on the pore size, structure and mechanical properties of the scaffolds were subsequently studied. In addition, the mass loss, the swelling ratio and the pH of the scaffolds were determined following their immersion in a cell culture medium. Furthermore, the metabolic activity of human mesenchymal stem cells (hMSCs) cultivated in scaffold infusions for 2 and 7 days was assessed. Finally, studies were conducted of cell adhesion, proliferation and penetration into the scaffolds. With regard to the structural stability of the tested scaffolds, it was determined that EDC/NHS/PBS and genipin formed the most effectively cross-linked materials. Moreover, it was discovered that the genipin cross-linked scaffold also provided the best conditions for hMSC cultivation. In addition, the infusions from all the scaffolds were found to be non-cytotoxic. Thus, the genipin and EDC/NHS/PBS cross-linked scaffolds can be considered to be promising biomaterials for further in vivo testing and bone surgery applications.

Citace poskytuje Crossref.org

Nejnovějších 20 citací...

Zobrazit více v
Medvik | PubMed

Comparative Study of the Dehydrothermal Crosslinking of Electrospun Collagen Nanofibers: The Effects of Vacuum Conditions and Subsequent Chemical Crosslinking

. 2024 Aug 29 ; 16 (17) : . [epub] 20240829

Enhancing Guided Bone Regeneration with a Novel Carp Collagen Scaffold: Principles and Applications

. 2024 Jun 01 ; 15 (6) : . [epub] 20240601

Various Simulated Body Fluids Lead to Significant Differences in Collagen Tissue Engineering Scaffolds

. 2021 Aug 05 ; 14 (16) : . [epub] 20210805

Vancomycin-releasing cross-linked collagen sponges as wound dressings

. 2021 Feb 01 ; 21 (1) : 61-70. [epub] 20210201

Rifampin-Releasing Triple-Layer Cross-Linked Fresh Water Fish Collagen Sponges as Wound Dressings

. 2020 ; 2020 () : 3841861. [epub] 20201016

Positive impact of dynamic seeding of mesenchymal stem cells on bone-like biodegradable scaffolds with increased content of calcium phosphate nanoparticles

. 2019 Aug ; 46 (4) : 4483-4500. [epub] 20190610

Note on the use of different approaches to determine the pore sizes of tissue engineering scaffolds: what do we measure?

. 2018 Aug 17 ; 17 (1) : 110. [epub] 20180817

Dry versus hydrated collagen scaffolds: are dry states representative of hydrated states?

. 2018 Feb 01 ; 29 (3) : 20. [epub] 20180201

Najít záznam

Citační ukazatele

Nahrávání dat ...

Možnosti archivace

Nahrávání dat ...