The effects of different cross-linking conditions on collagen-based nanocomposite scaffolds-an in vitro evaluation using mesenchymal stem cells
Language English Country Great Britain, England Media electronic
Document type Comparative Study, Evaluation Study, Journal Article, Research Support, Non-U.S. Gov't
- MeSH
- Equipment Failure Analysis MeSH
- Biocompatible Materials chemical synthesis MeSH
- Cell Adhesion physiology MeSH
- Equipment Design MeSH
- Collagen chemistry MeSH
- Bone Matrix chemistry MeSH
- Bone Substitutes chemical synthesis MeSH
- Humans MeSH
- Mesenchymal Stem Cells cytology physiology MeSH
- Nanocomposites chemistry ultrastructure MeSH
- Cell Proliferation physiology MeSH
- Cross-Linking Reagents chemistry MeSH
- Materials Testing MeSH
- Tissue Scaffolds * MeSH
- Mesenchymal Stem Cell Transplantation instrumentation methods MeSH
- Particle Size MeSH
- Cell Survival physiology MeSH
- Check Tag
- Humans MeSH
- Publication type
- Journal Article MeSH
- Evaluation Study MeSH
- Research Support, Non-U.S. Gov't MeSH
- Comparative Study MeSH
- Names of Substances
- Biocompatible Materials MeSH
- Collagen MeSH
- Bone Substitutes MeSH
- Cross-Linking Reagents MeSH
Nanocomposite scaffolds which aimed to imitate a bone extracellular matrix were prepared for bone surgery applications. The scaffolds consisted of polylactide electrospun nano/sub-micron fibres, a natural collagen matrix supplemented with sodium hyaluronate and natural calcium phosphate nano-particles (bioapatite). The mechanical properties of the scaffolds were improved by means of three different cross-linking agents: N-(3-dimethylamino propyl)-N'-ethylcarbodiimide hydrochloride and N-hydroxysuccinimide in an ethanol solution (EDC/NHS/EtOH), EDC/NHS in a phosphate buffer saline solution (EDC/NHS/PBS) and genipin. The effect of the various cross-linking conditions on the pore size, structure and mechanical properties of the scaffolds were subsequently studied. In addition, the mass loss, the swelling ratio and the pH of the scaffolds were determined following their immersion in a cell culture medium. Furthermore, the metabolic activity of human mesenchymal stem cells (hMSCs) cultivated in scaffold infusions for 2 and 7 days was assessed. Finally, studies were conducted of cell adhesion, proliferation and penetration into the scaffolds. With regard to the structural stability of the tested scaffolds, it was determined that EDC/NHS/PBS and genipin formed the most effectively cross-linked materials. Moreover, it was discovered that the genipin cross-linked scaffold also provided the best conditions for hMSC cultivation. In addition, the infusions from all the scaffolds were found to be non-cytotoxic. Thus, the genipin and EDC/NHS/PBS cross-linked scaffolds can be considered to be promising biomaterials for further in vivo testing and bone surgery applications.
References provided by Crossref.org
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