The plant hormones cytokinins are a convenient target of genetic manipulations that bring benefits in biotechnological applications. The present work demonstrates the importance of the subcellular compartmentalization of cytokinins on the model dicot plant Arabidopsis thaliana and monocot crop Hordeum vulgare. The method of protoplast and vacuole isolation combined with precise cytokinin analysis and recovery assay of a vacuolar marker protein were used to quantify the contents of individual cytokinin forms in the leaf extracellular space, cell interior and vacuole. The data obtained for wild type plants and in each case a specific mutant line allow comparing the effect of genetic manipulations on the hormone distribution and homeostatic balance of cytokinins in the modified plants.

Department of Metabolomics Centre of the Region Haná for Biotechnological and Agricultural Research Institute of Experimental Botany AS CR Šlechtitelů 27 Olomouc CZ 78371 Czech Republic

Department of Molecular Biology Centre of the Region Haná for Biotechnological and Agricultural Research Faculty of Science Palacký University Šlechtitelů 27 Olomouc CZ 78371 Czech Republic

Department of Molecular Biology Centre of the Region Haná for Biotechnological and Agricultural Research Faculty of Science Palacký University Šlechtitelů 27 Olomouc CZ 78371 Czech Republic; Umeå Plant Science Center Department of Forest Genetics and Plant Physiology Swedish University of Agricultural Sciences Umeå SE 90183 Sweden

Umeå Plant Science Center Department of Forest Genetics and Plant Physiology Swedish University of Agricultural Sciences Umeå SE 90183 Sweden

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