The plant hormones cytokinins are a convenient target of genetic manipulations that bring benefits in biotechnological applications. The present work demonstrates the importance of the subcellular compartmentalization of cytokinins on the model dicot plant Arabidopsis thaliana and monocot crop Hordeum vulgare. The method of protoplast and vacuole isolation combined with precise cytokinin analysis and recovery assay of a vacuolar marker protein were used to quantify the contents of individual cytokinin forms in the leaf extracellular space, cell interior and vacuole. The data obtained for wild type plants and in each case a specific mutant line allow comparing the effect of genetic manipulations on the hormone distribution and homeostatic balance of cytokinins in the modified plants.

Department of Metabolomics Centre of the Region Haná for Biotechnological and Agricultural Research Institute of Experimental Botany AS CR Šlechtitelů 27 Olomouc CZ 78371 Czech Republic

Department of Molecular Biology Centre of the Region Haná for Biotechnological and Agricultural Research Faculty of Science Palacký University Šlechtitelů 27 Olomouc CZ 78371 Czech Republic

Department of Molecular Biology Centre of the Region Haná for Biotechnological and Agricultural Research Faculty of Science Palacký University Šlechtitelů 27 Olomouc CZ 78371 Czech Republic; Umeå Plant Science Center Department of Forest Genetics and Plant Physiology Swedish University of Agricultural Sciences Umeå SE 90183 Sweden

Umeå Plant Science Center Department of Forest Genetics and Plant Physiology Swedish University of Agricultural Sciences Umeå SE 90183 Sweden

References provided by Crossref.org

Auxin Metabolome Profiling in the Arabidopsis Endoplasmic Reticulum Using an Optimised Organelle Isolation Protocol

Integrating the Roles for Cytokinin and Auxin in De Novo Shoot Organogenesis: From Hormone Uptake to Signaling Outputs

Differential Subcellular Distribution of Cytokinins: How Does Membrane Transport Fit into the Big Picture?

The Hulks and the Deadpools of the Cytokinin Universe: A Dual Strategy for Cytokinin Production, Translocation, and Signal Transduction

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trans-Zeatin-N-glucosides have biological activity in Arabidopsis thaliana

Auxins and Cytokinins-The Role of Subcellular Organization on Homeostasis

Profiles of Endogenous Phytohormones Over the Course of Norway Spruce Somatic Embryogenesis

Plant Hormonomics: Multiple Phytohormone Profiling by Targeted Metabolomics

The Cytokinin Oxidase/Dehydrogenase CKX1 Is a Membrane-Bound Protein Requiring Homooligomerization in the Endoplasmic Reticulum for Its Cellular Activity

Purification of Maize Nucleotide Pyrophosphatase/Phosphodiesterase Casts Doubt on the Existence of Zeatin Cis-Trans Isomerase in Plants

Cytokinin-Specific Glycosyltransferases Possess Different Roles in Cytokinin Homeostasis Maintenance