Reduced Levels of Tissue Inhibitors of Metalloproteinases in UVB-Irradiated Corneal Epithelium
Language English Country United States Media print-electronic
Document type Journal Article, Research Support, Non-U.S. Gov't
PubMed
27317620
DOI
10.1111/php.12612
Knihovny.cz E-resources
- MeSH
- Immunohistochemistry MeSH
- Rabbits MeSH
- Real-Time Polymerase Chain Reaction MeSH
- Matrix Metalloproteinases genetics metabolism MeSH
- RNA, Messenger genetics metabolism MeSH
- Epithelium, Corneal enzymology radiation effects MeSH
- Ultraviolet Rays * MeSH
- Animals MeSH
- Check Tag
- Rabbits MeSH
- Animals MeSH
- Publication type
- Journal Article MeSH
- Research Support, Non-U.S. Gov't MeSH
- Names of Substances
- Matrix Metalloproteinases MeSH
- RNA, Messenger MeSH
Tissue inhibitors of metalloproteinases (TIMPs) are the major endogenous regulators of metalloproteinase activity in tissues. TIMPs are able to inhibit activity of all known matrix metalloproteinases (MMPs) and thus participate in controlling extracellular matrix synthesis and degradation. We showed previously elevated expressions of MMPs in the rabbit corneal epithelium upon UVB exposure and suggested that these enzymes might be involved in corneal destruction caused by excessive proteolysis. The aim of this study was to investigate TIMPs in the corneal epithelium after UV irradiation using immunohistochemical and biochemical methods. We found that as compared to control rabbit corneas where relatively high levels of TIMPs were present in the epithelium, repeated irradiation of the cornea with UVB rays (not with UVA rays of similar doses) significantly decreased TIMPs in corneal epithelial cells. The results of this study point to the suggestion that the decrease in TIMPs in the corneal epithelium after UVB irradiation contributes to increased proteolytic activity of MMPs in UVB-irradiated corneal epithelium found previously.
Faculty Hospital Královské Vinohrady Prague Czech Republic
Institute of Experimental Medicine Academy of Sciences of the Czech Republic Prague Czech Republic
References provided by Crossref.org
The Healing of Oxidative Injuries with Trehalose in UVB-Irradiated Rabbit Corneas
GENBANK
NM_001082253, NM_001195690, XM_008252508
