Targeting distinct myeloid cell populations in vivo using polymers, liposomes and microbubbles
Language English Country Netherlands Media print-electronic
Document type Journal Article, Research Support, Non-U.S. Gov't
PubMed
27855336
DOI
10.1016/j.biomaterials.2016.11.009
PII: S0142-9612(16)30612-3
Knihovny.cz E-resources
- Keywords
- Liposomes, Macrophages, Microbubbles, Nanomedicine, Polymers, Targeted delivery,
- MeSH
- Molecular Targeted Therapy methods MeSH
- Liposomes chemistry MeSH
- Microbubbles therapeutic use MeSH
- Myeloid Cells chemistry cytology MeSH
- Mice, Nude MeSH
- Mice MeSH
- Nanocapsules administration & dosage chemistry MeSH
- Organ Specificity MeSH
- Polymers chemistry MeSH
- Materials Testing MeSH
- Tissue Distribution MeSH
- Capsules administration & dosage chemistry MeSH
- Viscera chemistry cytology MeSH
- Animals MeSH
- Check Tag
- Mice MeSH
- Animals MeSH
- Publication type
- Journal Article MeSH
- Research Support, Non-U.S. Gov't MeSH
- Names of Substances
- Liposomes MeSH
- Nanocapsules MeSH
- Polymers MeSH
- Capsules MeSH
Identifying intended or accidental cellular targets for drug delivery systems is highly relevant for evaluating therapeutic and toxic effects. However, limited knowledge exists on the distribution of nano- and micrometer-sized carrier systems at the cellular level in different organs. We hypothesized that clinically relevant carrier materials, differing in composition and size, are able to target distinct myeloid cell subsets that control inflammatory processes, such as macrophages, neutrophils, monocytes and dendritic cells. Therefore, we analyzed the biodistribution and in vivo cellular uptake of intravenously injected poly(N-(2-hydroxypropyl) methacrylamide) polymers, PEGylated liposomes and poly(butyl cyanoacrylate) microbubbles in mice, using whole-body imaging (computed tomography - fluorescence-mediated tomography), intra-organ imaging (intravital multi-photon microscopy) and cellular analysis (flow cytometry of blood, liver, spleen, lung and kidney). While the three carrier materials shared accumulation in tissue macrophages in liver and spleen, they notably differed in uptake by other myeloid subsets. Kupffer cells and splenic red pulp macrophages rapidly take up microbubbles. Liposomes efficiently reach dendritic cells in liver, lung and kidney. Polymers exhibit the longest circulation half-life and target endothelial cells in the liver, neutrophils and alveolar macrophages. The identification of such previously unrecognized target cell populations might open up new avenues for more efficient drug delivery.
Department of Medicine 3 University Hospital Aachen Aachen Germany
Department of Medicine 3 University Hospital Hamburg Eppendorf Hamburg Germany
Institute of Macromolecular Chemistry Academy of Sciences of the Czech Republic Prague Czechia
Institute of Organic Chemistry Johannes Gutenberg University Mainz Germany
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