Electrochemical Determination of the Antioxidant Potential of Some Less Common Fruit Species
Status PubMed-not-MEDLINE Language English Country Switzerland Media electronic
Document type Journal Article
PubMed
27873945
PubMed Central
PMC3790976
DOI
10.3390/s8127564
PII: s8127564
Knihovny.cz E-resources
- Keywords
- Antioxidant Capacity, DPPH• test, Flavonoids, Less Common Fruit Species, Liquid Chromatography with Electrochemical Detection,
- Publication type
- Journal Article MeSH
Various berries and fruit types of less common fruit species are known to contain antioxidants. Consumption of high amounts of antioxidant flavonoids, which display a variety of biological properties, including antiproliferative and anti-inflammatory activity, may have a positive impact on human health, particularly for the prevention of cancer and other inflammatory diseases. In these studies, based on the hypothesis that the fruit extract with the highest content would possess significantly higher health benefits, flavonoid-rich extracts were obtained from some less common fruit species - Blue Honeysuckles (Lonicera Kamtschatica and Lonicera edulis, Turcz. ex. Freyn), Saskatoon berry (Amelanchier alnifolia Nutt.) and Chinese Hawthorn (Crataegus pinnatifida BUNGE) - grown from germplasm held at the Mendel University of Agriculture and Forestry in Brno, Czech Republic and then characterized in terms of biological value based on the results from a relative antioxidant capacity assessment. The antioxidant content evaluation was based on the total flavonoid amount, determined by liquid chromatography with electrochemical detection (HPLC-ED). A DPPH• test was applied as a reference. The antioxidant content measured in Chinese Hawthorn fruit extract identified it as a potent source of flavonoid antioxidants, with a content 9-fold higher than that seen in Amelanchier fruit. The multifunctional HPLC-ED array method coupled with a DPPH• reference appears to be the optimal analytical progress, accurately reflecting the nutritivetherapeutic properties of a fruit.
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Gey K.F. The Antioxidant Hypothesis of Cardiovascular-Disease - Epidemiology and Mechanisms. Biochem. Soc. Trans. 1990;18:1041–1045. PubMed
Gillman M.W., Cupples L.A., Gagnon D., Posner B.M., Ellison R.C., Castelli W.P., Wolf P.A. Protective Effect of Fruits and Vegetables on Development of Stroke in Men. JAMA. 1995;273:1113–1117. PubMed
Hodek P., Hanustiak P., Krizkova J., Mikelova R., Krizkova S., Stiborova M., Trnkova L., Horna A., Beklova M., Kizek R. Toxicological aspects of flavonoid interaction with biomacromolecules. Neuroendocrinol. Lett. 2006;27:14–17. PubMed
Gey K.F., Puska P., Jordan P., Moser U.K. Inverse Correlation between Plasma Vitamin-E and Mortality from Ischemic-Heart-Disease in Cross-Cultural Epidemiology. Am. J. Clin. Nutr. 1991;53:S326–S334. PubMed
La Vecchia C., Altieri A., Tavani A. Vegetables, fruit, antioxidants and cancer: a review of Italian studies. Eur. J. Nutr. 2001;40:261–267. PubMed
Rickman J.C., Barrett D.M., Bruhn C.M. Nutritional comparison of fresh, frozen and canned fruits and vegetables. Part 1. Vitamins C and B and phenolic compounds. J. Sci. Food Agric. 2007;87:930–944.
Lau F.C., Shukitt-Hale B., Joseph J.A. Beneficial effects of berry fruit polyphenols on neuronal and behavioral aging. J. Sci. Food Agric. 2006;86:2251–2255.
Barberousse H., Roiseux O., Robert C., Paquot M., Deroanne C., Blecker C. Analytical methodologies for quantification of ferulic acid and its oligomers. J. Sci. Food Agric. 2008;88:1494–1511.
Jac P., Polasek M., Pospisilova M. Recent trends in the determination of polyphenols by electromigration methods. J. Pharm. Biomed. Anal. 2006;40:805–814. PubMed
Long H., Zhu Y.X., Coury L.A., Duda C.T., Kissinger C.B., Kissinger P.T. Simultaneous multiple-electrode liquid chromatography-electrochemistry of phenolic acids in honey. Lc. Gc. N. Am. 2002:61–64.
Pellegrini N., Del Rio D., Colombi B., Bianchi M., Brighenti F. Application of the 2, 2 ′-azinobis(3-ethylbenzothiazoline-6-sulfonic acid) radical cation assay to a flow injection system for the evaluation of antioxidant activity of some pure compounds and beverages. J. Agric. Food Chem. 2003;51:260–264. PubMed
Willett W.C. Micronutrients and Cancer Risk. Am. J. Clin. Nutr. 1994;59:S1162–S1165. PubMed
Benzie I.F.F., Strain J.J. Ferric reducing antioxidant power assay: Direct measure of total antioxidant activity of biological fluids and modified version for simultaneous measurement of total antioxidant power and ascorbic acid concentration. Meth. Enzymol. 1999;277:15–27. PubMed
Wang H., Cao G.H., Prior R.L. Total antioxidant capacity of fruits. J. Agric. Food Chem. 1996;44:701–705.
Riceevans C.A., Miller N.J., Bolwell P.G., Bramley P.M., Pridham J.B. The Relative Antioxidant Activities of Plant-Derived Polyphenolic Flavonoids. Free Radic. Res. 1995;22:375–383. PubMed
Hakkinen S., Heinonen M., Karenlampi S., Mykkanen H., Ruuskanen J., Torronen R. Screening of selected flavonoids and phenolic acids in 19 berries. Food Res. Int. 1999;32:345–353.
Ercisli S. Chemical composition of fruits in some rose (Rosa spp.) species. Food Chem. 2007;104:1379–1384.
SatueGracia M.T., Heinonen M., Frankel E.N. Anthocyanins as antioxidants on human low-density lipoprotein and lecithin-liposome systems. J. Agric. Food Chem. 1997;45:3362–3367.
Effect of five different stages of ripening on chemical compounds in medlar (Mespilus germanica L.)
Content of phenolic compounds and antioxidant capacity in fruits of apricot genotypes
