Extracellular Matrix Hydrogel Derived from Human Umbilical Cord as a Scaffold for Neural Tissue Repair and Its Comparison with Extracellular Matrix from Porcine Tissues
Language English Country United States Media print-electronic
Document type Comparative Study, Journal Article
- Keywords
- extracellular matrix, hydrogel, mesenchymal stromal cells, neural tissue, regeneration, umbilical cord,
- MeSH
- Species Specificity MeSH
- Extracellular Matrix chemistry MeSH
- Hydrogels chemistry MeSH
- Humans MeSH
- Mesenchymal Stem Cells cytology metabolism MeSH
- Nerve Tissue cytology metabolism MeSH
- Cell Movement MeSH
- Swine MeSH
- Cell Proliferation MeSH
- Umbilical Cord chemistry MeSH
- Tissue Scaffolds economics MeSH
- Animals MeSH
- Check Tag
- Humans MeSH
- Animals MeSH
- Publication type
- Journal Article MeSH
- Comparative Study MeSH
- Names of Substances
- Hydrogels MeSH
Extracellular matrix (ECM) hydrogels prepared by tissue decellularization have been reported as natural injectable materials suitable for neural tissue repair. In this study, we prepared ECM hydrogel derived from human umbilical cord (UC) and evaluated its composition and mechanical and biological properties in comparison with the previously described ECM hydrogels derived from porcine urinary bladder (UB), brain, and spinal cord. The ECM hydrogels did not differ from each other in the concentration of collagen, while the highest content of glycosaminoglycans as well as the shortest gelation time was found for UC-ECM. The elastic modulus was then found to be the highest for UB-ECM. In spite of a different origin, topography, and composition, all ECM hydrogels similarly promoted the migration of human mesenchymal stem cells (MSCs) and differentiation of neural stem cells, as well as axonal outgrowth in vitro. However, only UC-ECM significantly improved proliferation of tissue-specific UC-derived MSCs when compared with the other ECMs. Injection of UC-ECM hydrogels into a photothrombotic cortical ischemic lesion in rats proved its in vivo gelation and infiltration with host macrophages. In summary, this study proposes UC-ECM hydrogel as an easily accessible biomaterial of human origin, which has the potential for neural as well as other soft tissue reconstruction.
2nd Medical Faculty Charles University Prague Czech Republic
Institute of Experimental Medicine Academy of Sciences of the Czech Republic Prague Czech Republic
Institute of Physics Academy of Sciences of the Czech Republic Prague Czech Republic
References provided by Crossref.org
Mesenchymal Stem Cells in Treatment of Spinal Cord Injury and Amyotrophic Lateral Sclerosis
Biomaterials and Magnetic Stem Cell Delivery in the Treatment of Spinal Cord Injury
