Differentiation between Staphylococcus aureus and Staphylococcus epidermidis strains using Raman spectroscopy
Language English Country Great Britain, England Media print-electronic
Document type Journal Article, Research Support, Non-U.S. Gov't
PubMed
28686040
DOI
10.2217/fmb-2016-0224
Knihovny.cz E-resources
- Keywords
- Raman spectroscopy, Staphylococcus epidermidis, Staphyococcus aureus, rapid diagnostics,
- MeSH
- Bacteriological Techniques MeSH
- Humans MeSH
- Colony Count, Microbial methods MeSH
- Spectrum Analysis, Raman methods MeSH
- Staphylococcal Infections diagnosis MeSH
- Staphylococcus aureus isolation & purification pathogenicity MeSH
- Staphylococcus epidermidis isolation & purification pathogenicity MeSH
- Check Tag
- Humans MeSH
- Publication type
- Journal Article MeSH
- Research Support, Non-U.S. Gov't MeSH
- Geographicals
- Czech Republic MeSH
AIM: Raman spectroscopy is an analytical method with a broad range of applications across multiple scientific fields. We report on a possibility to differentiate between two important Gram-positive species commonly found in clinical material - Staphylococcus aureus and Staphylococcus epidermidis - using this rapid noninvasive technique. MATERIALS & METHODS: For this, we tested 87 strains, 41 of S. aureus and 46 of S. epidermidis, directly from colonies grown on a Mueller-Hinton agar plate using Raman spectroscopy. DISCUSSION & CONCLUSION: The method paves a way for separation of these two species even on high number of samples and therefore, it can be potentially used in clinical diagnostics.
References provided by Crossref.org
