Comprehensive assessment of electrospun scaffolds hemocompatibility
Language English Country Netherlands Media print-electronic
Document type Journal Article
PubMed
29025666
DOI
10.1016/j.msec.2017.05.011
PII: S0928-4931(16)30791-3
Knihovny.cz E-resources
- Keywords
- Blood compatibility, Collagen, Copolymer of polylactide and polycaprolactone, Fibrous scaffolds, Polycaprolactone,
- MeSH
- Biocompatible Materials chemical synthesis chemistry pharmacology MeSH
- Hemolysis drug effects MeSH
- Collagen chemistry MeSH
- Blood Cells cytology drug effects metabolism MeSH
- Humans MeSH
- Microscopy, Electron, Scanning MeSH
- Partial Thromboplastin Time MeSH
- Polyesters chemistry MeSH
- Polymers chemical synthesis chemistry MeSH
- Prothrombin Time MeSH
- Check Tag
- Humans MeSH
- Publication type
- Journal Article MeSH
- Names of Substances
- Biocompatible Materials MeSH
- Collagen MeSH
- poly(lactide) MeSH Browser
- polycaprolactone MeSH Browser
- Polyesters MeSH
- Polymers MeSH
Biodegradable polyesters, namely polycaprolactone (PCL) and copolymer of polylactide and polycaprolactone (PLCL) were electrospun into various fibrous structures and their hemocompatibility was evaluated in vitro. Firstly, hemolytic effect was evaluated upon incubation with diluted whole blood. The results showed that the degree of hemolysis depended on chemical composition and fibrous morphology. Electrospun polycaprolactone induced slight degree of hemolysis depending on its molecular weight and fibrous morphology; copolymer PLCL did not cause detectable hemolysis. The influence of coagulation pathways was examined by measurement of coagulation times. It was showed that intrinsic coagulation pathway assessed by activated partial thromboplastin time (APTT) was moderately accelerated after incubation with PCL and prolonged after incubation with copolymer PLCL. Extrinsic activation of coagulation tested by prothrombin time (PT) was slightly accelerated after incubation with all tested electrospun samples. Thrombogenicity assessment of fibrous samples revealed high thrombogenic properties of fibrous materials that was comparable to high degree of collagen thrombogenicity. The level of platelet activation was dependent on chemical composition and surface morphology of tested materials.
Liberec Regional Hospital Department of Blood Transfusion Baarova 15 460 01 Liberec Czech Republic
Liberec Regional Hospital Department of Clinical Hematology Baarova 15 460 01 Liberec Czech Republic
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