The study describes the detailed examination of the effect of ethylene oxide sterilization on electrospun scaffolds constructed from biodegradable polyesters. Different fibrous layers fabricated from polycaprolactone (PCL) and a copolymer consisting of polylactide and polycaprolactone (PLCL) were investigated for the determination of their mechanical properties, degradation rates and interaction with fibroblasts. It was discovered that the sterilization procedure influenced the mechanical properties of the electrospun PLCL copolymer scaffold to the greatest extent. No effect of ethylene oxide sterilization on degradation behavior was observed. However, a delayed fibroblast proliferation rate was noticed with concern to the ethylene oxide sterilized samples compared to the ethanol sterilization of the materials.

• MeSH
• biokompatibilní materiály chemie   metabolismus   farmakologie   MeSH
• buněčné linie MeSH
• cévní protézy MeSH
• ethylenoxid chemie   farmakologie   MeSH
• mikroskopie elektronová rastrovací MeSH
• modul pružnosti MeSH
• myši MeSH
• nanovlákna chemie   MeSH
• pevnost v tahu MeSH
• polyestery chemie   metabolismus   MeSH
• sterilizace MeSH
• viabilita buněk účinky léků   MeSH
• zvířata MeSH
• Check Tag
• myši MeSH
• zvířata MeSH
• Publikační typ
• časopisecké články MeSH

Biodegradable polyesters, namely polycaprolactone (PCL) and copolymer of polylactide and polycaprolactone (PLCL) were electrospun into various fibrous structures and their hemocompatibility was evaluated in vitro. Firstly, hemolytic effect was evaluated upon incubation with diluted whole blood. The results showed that the degree of hemolysis depended on chemical composition and fibrous morphology. Electrospun polycaprolactone induced slight degree of hemolysis depending on its molecular weight and fibrous morphology; copolymer PLCL did not cause detectable hemolysis. The influence of coagulation pathways was examined by measurement of coagulation times. It was showed that intrinsic coagulation pathway assessed by activated partial thromboplastin time (APTT) was moderately accelerated after incubation with PCL and prolonged after incubation with copolymer PLCL. Extrinsic activation of coagulation tested by prothrombin time (PT) was slightly accelerated after incubation with all tested electrospun samples. Thrombogenicity assessment of fibrous samples revealed high thrombogenic properties of fibrous materials that was comparable to high degree of collagen thrombogenicity. The level of platelet activation was dependent on chemical composition and surface morphology of tested materials.

• MeSH
• biokompatibilní materiály chemická syntéza   chemie   farmakologie   MeSH
• hemolýza účinky léků   MeSH
• kolagen chemie   MeSH
• krevní buňky cytologie   účinky léků   metabolismus   MeSH
• lidé MeSH
• mikroskopie elektronová rastrovací MeSH
• parciální tromboplastinový čas MeSH
• polyestery chemie   MeSH
• polymery chemická syntéza   chemie   MeSH
• protrombinový čas MeSH
• Check Tag
• lidé MeSH
• Publikační typ
• časopisecké články MeSH

The control of the surface properties is an important issue for applicability of polymer membranes interacting with cells. In this work, the influence of surface roughness and stiffness of two polymer membranes on viability and mechanical properties of keratinocytes was studied. Terpolimer polyglicolide, polycaprolactone and polylactide, (PGA-PCL-PLA) and copolymer polycaprolactone, polyglicolide (PGA-PCL) substrates were used for membranes fabrication. Surface modification - the hydrolysis of the obtained membranes was carried out. The analysis of membranes' surface properties revealed that RMS surface roughness and roughness factor of PGA-PCL-PLA membrane decreased after hydrolysis while its stiffness increased. In contrast, the PGA-PCL membrane stiffness was only slightly affected by NaOH treatment. Immortalized human keratinocytes (HaCaT) were grown under standard conditions on the surface of the studied membranes and characterized by means of atomic force microscopy and fluorescence microcopy. The results showed the substrate-dependent effect on cells' properties.

• MeSH
• buněčné linie MeSH
• fluorescenční mikroskopie MeSH
• keratinocyty účinky léků   fyziologie   MeSH
• lidé MeSH
• membrány chemie   MeSH
• mikroskopie atomárních sil MeSH
• polymery chemická syntéza   toxicita   MeSH
• povrchové vlastnosti * MeSH
• testování materiálů * MeSH
• viabilita buněk MeSH
• Check Tag
• lidé MeSH
• Publikační typ
• časopisecké články MeSH