Comprehensive assessment of electrospun scaffolds hemocompatibility
Jazyk angličtina Země Nizozemsko Médium print-electronic
Typ dokumentu časopisecké články
PubMed
29025666
DOI
10.1016/j.msec.2017.05.011
PII: S0928-4931(16)30791-3
Knihovny.cz E-zdroje
- Klíčová slova
- Blood compatibility, Collagen, Copolymer of polylactide and polycaprolactone, Fibrous scaffolds, Polycaprolactone,
- MeSH
- biokompatibilní materiály chemická syntéza chemie farmakologie MeSH
- hemolýza účinky léků MeSH
- kolagen chemie MeSH
- krevní buňky cytologie účinky léků metabolismus MeSH
- lidé MeSH
- mikroskopie elektronová rastrovací MeSH
- parciální tromboplastinový čas MeSH
- polyestery chemie MeSH
- polymery chemická syntéza chemie MeSH
- protrombinový čas MeSH
- Check Tag
- lidé MeSH
- Publikační typ
- časopisecké články MeSH
- Názvy látek
- biokompatibilní materiály MeSH
- kolagen MeSH
- poly(lactide) MeSH Prohlížeč
- polycaprolactone MeSH Prohlížeč
- polyestery MeSH
- polymery MeSH
Biodegradable polyesters, namely polycaprolactone (PCL) and copolymer of polylactide and polycaprolactone (PLCL) were electrospun into various fibrous structures and their hemocompatibility was evaluated in vitro. Firstly, hemolytic effect was evaluated upon incubation with diluted whole blood. The results showed that the degree of hemolysis depended on chemical composition and fibrous morphology. Electrospun polycaprolactone induced slight degree of hemolysis depending on its molecular weight and fibrous morphology; copolymer PLCL did not cause detectable hemolysis. The influence of coagulation pathways was examined by measurement of coagulation times. It was showed that intrinsic coagulation pathway assessed by activated partial thromboplastin time (APTT) was moderately accelerated after incubation with PCL and prolonged after incubation with copolymer PLCL. Extrinsic activation of coagulation tested by prothrombin time (PT) was slightly accelerated after incubation with all tested electrospun samples. Thrombogenicity assessment of fibrous samples revealed high thrombogenic properties of fibrous materials that was comparable to high degree of collagen thrombogenicity. The level of platelet activation was dependent on chemical composition and surface morphology of tested materials.
Liberec Regional Hospital Department of Blood Transfusion Baarova 15 460 01 Liberec Czech Republic
Liberec Regional Hospital Department of Clinical Hematology Baarova 15 460 01 Liberec Czech Republic
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