Cardiovascular disease predictors and adipose tissue macrophage polarization: Is there a link?
Language English Country Great Britain, England Media print-electronic
Document type Comparative Study, Journal Article, Research Support, Non-U.S. Gov't
- Keywords
- Cardiovascular risk factors, adipose tissue, inflammation, macrophage, statins,
- MeSH
- Biomarkers blood MeSH
- Adult MeSH
- Phagocytosis MeSH
- Phenotype MeSH
- Hypercholesterolemia blood drug therapy pathology MeSH
- Body Mass Index MeSH
- Middle Aged MeSH
- Humans MeSH
- Macrophages drug effects metabolism pathology MeSH
- Inflammation Mediators blood MeSH
- Intra-Abdominal Fat drug effects metabolism pathology MeSH
- Peripheral Arterial Disease blood pathology surgery MeSH
- Subcutaneous Fat drug effects metabolism pathology MeSH
- Prognosis MeSH
- Risk Factors MeSH
- Sex Factors MeSH
- Hydroxymethylglutaryl-CoA Reductase Inhibitors therapeutic use MeSH
- Case-Control Studies MeSH
- Age Factors MeSH
- Check Tag
- Adult MeSH
- Middle Aged MeSH
- Humans MeSH
- Male MeSH
- Female MeSH
- Publication type
- Journal Article MeSH
- Research Support, Non-U.S. Gov't MeSH
- Comparative Study MeSH
- Names of Substances
- Biomarkers MeSH
- Inflammation Mediators MeSH
- Hydroxymethylglutaryl-CoA Reductase Inhibitors MeSH
Background The risk of cardiovascular disease is closely connected to adipose tissue inflammation. The links between cardiovascular risk predictors and pro and anti-inflammatory macrophages in human adipose tissue were analysed to gain an insight into the pathophysiology of cardiovascular disease. Design Subcutaneous and visceral adipose tissues were obtained from 79 subjects, 52 living kidney donors (during nephrectomy) and 27 patients with peripheral artery disease (during arterial tree reconstruction). Methods Macrophage subsets were isolated from adipose tissues and analysed by flow cytometry using CD14, CD16, CD36 and CD163 monoclonal antibodies. The mutually adjusted differences of phagocytic pro-inflammatory (CD14 + CD16 + CD36high), anti-inflammatory (CD14 + CD16-CD163+) and transitional subsets of macrophages were analysed in relation to cardiovascular predictors (sex, age, body mass index, smoking, hypercholesterolaemia, hypertension and statin treatment). Results Age, male sex and hypercholesterolaemia were closely positively associated with the phagocytic pro-inflammatory macrophage subset in visceral adipose tissues. Interestingly, the proportion of phagocytic pro-inflammatory macrophages was relevantly decreased by statin therapy. A strong positive association of body mass index to the phagocytic pro-inflammatory subset was found in subcutaneous adipose tissues only. A minor transitional subpopulation, CD14 + CD16 + CD36lowCD163+, increased with age in both adipose tissues. This transitional subpopulation was also negatively associated with obesity and hypercholesterolaemia in visceral adipose tissues. Conclusion An effect of cardiovascular risk predictors on adipose tissue macrophage subpopulations was revealed. Interestingly, while age, male sex and hypercholesterolaemia were connected with the pro-inflammatory macrophage subpopulation in visceral adipose tissues, body mass index had a prominent effect in subcutaneous adipose tissues only. A decreasing effect of statins on these pro-inflammatory macrophages was documented.
2nd Faculty of Medicine Charles University Czech Republic
Anaesthesiology Resuscitation and Intensive Care Unit Military University Hospital Czech Republic
Department of Transplant Surgery Institute for Clinical and Experimental Medicine Czech Republic
References provided by Crossref.org
Phenotypic Shifts in Macrophages Within Advanced Atherosclerotic Plaques in Humans
Physiology and Pathobiology of Perivascular Adipose Tissue: Inflammation-based Atherogenesis
Rapid Drop in Coronary Heart Disease Mortality in Czech Male Population-What Was Actually behind It?
Cholesterol in the Cell Membrane-An Emerging Player in Atherogenesis
Adipose tissue macrophages and atherogenesis - a synergy with cholesterolaemia
The effect of cytokines produced by human adipose tissue on monocyte adhesion to the endothelium
