Limitations of routine MALDI-TOF mass spectrometric identification of Acinetobacter species and remedial actions
Language English Country Netherlands Media print-electronic
Document type Journal Article, Research Support, Non-U.S. Gov't
PubMed
30332615
DOI
10.1016/j.mimet.2018.10.009
PII: S0167-7012(18)30590-6
Knihovny.cz E-resources
- Keywords
- Acinetobacter spp., Bacteria profiling, BioTyper, MALDI-TOF MS,
- MeSH
- Acinetobacter classification genetics isolation & purification MeSH
- Genes, Bacterial MeSH
- Databases, Genetic MeSH
- Phylogeny MeSH
- Acinetobacter Infections diagnosis microbiology MeSH
- Limit of Detection * MeSH
- RNA, Ribosomal, 16S genetics MeSH
- Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization instrumentation methods MeSH
- Bacterial Typing Techniques instrumentation methods MeSH
- Publication type
- Journal Article MeSH
- Research Support, Non-U.S. Gov't MeSH
- Names of Substances
- RNA, Ribosomal, 16S MeSH
A set of 204 taxonomically well-defined strains belonging to 17 Acinetobacter spp., including 11 recently described species (A. albensis, A. bohemicus, A. colistiniresistens, A. courvalinii. A. dispersus, A. gandensis, A. modestus, A. proteolyticus, A. seifertii, A. variabilis, and A. vivianii) and six species of the so-called haemolytic clade (A. beijerinckii, A. gyllenbergii, A. haemolyticus, A. junii, A. parvus, and A. venetianus), were subjected to MALDI-TOF mass spectrometric profiling. The identification outputs were evaluated using the current version (8.0.0.0) of the commercially available Bruker Daltonics, Biotyper database, which does not contain reference entries for six of the species tested. Up to 29% of the strains were falsely identified as different Acinetobacter spp. present in the Biotyper database, resulting mostly from the close phylogenetic relationship of species of the haemolytic clade. To obtain more reliable identification, extending the commercial database showed only partial improvement, while the use of an alternative MALDI matrix solution (strongly acidified ferulic acid) allowed correct identification of nearly all problematic strains.
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