Effect of inactivation of luxS gene on the properties of Serratia proteamaculans 94 strain
Jazyk angličtina Země Spojené státy americké Médium print-electronic
Typ dokumentu časopisecké články
Grantová podpora
16-04-01048
Russian Foundation for Basic Research
18-04-00375
Russian Foundation for Basic Research
PubMed
30361877
DOI
10.1007/s12223-018-0657-5
PII: 10.1007/s12223-018-0657-5
Knihovny.cz E-zdroje
- MeSH
- bakteriální proteiny genetika MeSH
- biofilmy růst a vývoj MeSH
- fenotyp MeSH
- homoserin analogy a deriváty metabolismus MeSH
- laktony metabolismus MeSH
- lyasy štěpící vazby C-S genetika MeSH
- maso mikrobiologie MeSH
- mikrobiální interakce MeSH
- quorum sensing genetika MeSH
- regulace genové exprese u bakterií MeSH
- Serratia genetika metabolismus MeSH
- těkavé organické sloučeniny analýza MeSH
- umlčování genů * MeSH
- Publikační typ
- časopisecké články MeSH
- Názvy látek
- bakteriální proteiny MeSH
- homoserin MeSH
- laktony MeSH
- LuxS protein, Bacteria MeSH Prohlížeč
- lyasy štěpící vazby C-S MeSH
- N-octanoylhomoserine lactone MeSH Prohlížeč
- těkavé organické sloučeniny MeSH
The luxS gene is responsible for the synthesis of AI-2 (autoinducer-2), a signaling molecule that participates in quorum sensing regulation in a large number of bacteria. In this work, we investigated which phenotypes are regulated by luxS gene in Serratia proteamaculans 94, psychrotrophic strain isolated from spoiled refrigerated meat. AI-2 was identified in S. proteamaculans 94, and the luxS gene involved in its synthesis was cloned and sequenced. A mutant with the inactivated luxS gene was constructed. Inactivation of the luxS gene was shown to lead to the absence of AI-2 synthesis, chitinolytic activity, swimming motility, suppression of the growth of fungal plant pathogens Rhizoctonia solani and Helminthosporium sativum by volatile compounds emitted by S. proteamaculans 94 strain, and to a decrease of extracellular proteolytic activity. The knockout of the luxS gene did not affect synthesis of N-acyl-homoserine lactones, lipolytic, and hemolytic activities of S. proteamaculans 94.
Demidov Yaroslavl State University Sovetskaya Str 14 Yaroslavl Russia 150003
Institute of Molecular Genetics Russian Academy of Sciences Kurchatov sq 2 Moscow Russia 123182
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