Parallel in vitro and in silico investigations into anti-inflammatory effects of non-prenylated stilbenoids
Jazyk angličtina Země Anglie, Velká Británie Médium print-electronic
Typ dokumentu časopisecké články
Grantová podpora
T 942
Austrian Science Fund FWF - Austria
PubMed
30797367
DOI
10.1016/j.foodchem.2019.01.128
PII: S0308-8146(19)30203-1
Knihovny.cz E-zdroje
- Klíčová slova
- Anti-inflammatory, Cyclooxygenase, Lipoxygenase, Macrophages, Molecular docking simulations, NF-κB, Stilbenes,
- MeSH
- antiflogistika nesteroidní chemie farmakologie MeSH
- HCT116 buňky MeSH
- inhibitory cyklooxygenasy 2 chemie farmakologie MeSH
- inhibitory lipoxygenas chemie farmakologie MeSH
- lidé MeSH
- lipopolysacharidy farmakologie MeSH
- makrofágy účinky léků metabolismus MeSH
- NF-kappa B metabolismus MeSH
- preklinické hodnocení léčiv metody MeSH
- prenylace MeSH
- signální transdukce účinky léků MeSH
- simulace molekulového dockingu MeSH
- stilbeny chemie farmakologie MeSH
- TNF-alfa metabolismus MeSH
- transkripční faktor AP-1 metabolismus MeSH
- Check Tag
- lidé MeSH
- Publikační typ
- časopisecké články MeSH
- Názvy látek
- 3,3',4,5'-tetrahydroxystilbene MeSH Prohlížeč
- antiflogistika nesteroidní MeSH
- inhibitory cyklooxygenasy 2 MeSH
- inhibitory lipoxygenas MeSH
- lipopolysacharidy MeSH
- NF-kappa B MeSH
- pinostilbene MeSH Prohlížeč
- stilbeny MeSH
- TNF-alfa MeSH
- transkripční faktor AP-1 MeSH
Stilbenoids represent a large group of bioactive compounds, which occur in food and medicinal plants. Twenty-five stilbenoids were screened in vitro for their ability to inhibit COX-1, COX-2 and 5-LOX. Piceatannol and pinostilbene showed activity comparable to the zileuton and ibuprofen, respectively. The anti-inflammatory potential of stilbenoids was further evaluated using THP-1 human monocytic leukemia cell line. Tests of the cytotoxicity on the THP-1 and HCT116 cell lines showed very low toxic effects. The tested stilbenoids were evaluated for their ability to attenuate the LPS-stimulated activation of NF-κB/AP-1. Most of the tested substances reduced the activity of NF-κB/AP-1 and later attenuated the expression of TNF-α. The effects of selected stilbenoids were further investigated on inflammatory signaling pathways. Non-prenylated stilbenoids regulated attenuation of NF-ĸB/AP-1 activity upstream by inhibiting the phosphorylation of MAPKs. A docking study used to in silico analyze the tested compounds confirmed their interaction with NF-ĸB, COX-2 and 5-LOX.
Citace poskytuje Crossref.org
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