Parallel in vitro and in silico investigations into anti-inflammatory effects of non-prenylated stilbenoids
Language English Country Great Britain, England Media print-electronic
Document type Journal Article
Grant support
T 942
Austrian Science Fund FWF - Austria
PubMed
30797367
DOI
10.1016/j.foodchem.2019.01.128
PII: S0308-8146(19)30203-1
Knihovny.cz E-resources
- Keywords
- Anti-inflammatory, Cyclooxygenase, Lipoxygenase, Macrophages, Molecular docking simulations, NF-κB, Stilbenes,
- MeSH
- Anti-Inflammatory Agents, Non-Steroidal chemistry pharmacology MeSH
- HCT116 Cells MeSH
- Cyclooxygenase 2 Inhibitors chemistry pharmacology MeSH
- Lipoxygenase Inhibitors chemistry pharmacology MeSH
- Humans MeSH
- Lipopolysaccharides pharmacology MeSH
- Macrophages drug effects metabolism MeSH
- NF-kappa B metabolism MeSH
- Drug Evaluation, Preclinical methods MeSH
- Prenylation MeSH
- Signal Transduction drug effects MeSH
- Molecular Docking Simulation MeSH
- Stilbenes chemistry pharmacology MeSH
- Tumor Necrosis Factor-alpha metabolism MeSH
- Transcription Factor AP-1 metabolism MeSH
- Check Tag
- Humans MeSH
- Publication type
- Journal Article MeSH
- Names of Substances
- 3,3',4,5'-tetrahydroxystilbene MeSH Browser
- Anti-Inflammatory Agents, Non-Steroidal MeSH
- Cyclooxygenase 2 Inhibitors MeSH
- Lipoxygenase Inhibitors MeSH
- Lipopolysaccharides MeSH
- NF-kappa B MeSH
- pinostilbene MeSH Browser
- Stilbenes MeSH
- Tumor Necrosis Factor-alpha MeSH
- Transcription Factor AP-1 MeSH
Stilbenoids represent a large group of bioactive compounds, which occur in food and medicinal plants. Twenty-five stilbenoids were screened in vitro for their ability to inhibit COX-1, COX-2 and 5-LOX. Piceatannol and pinostilbene showed activity comparable to the zileuton and ibuprofen, respectively. The anti-inflammatory potential of stilbenoids was further evaluated using THP-1 human monocytic leukemia cell line. Tests of the cytotoxicity on the THP-1 and HCT116 cell lines showed very low toxic effects. The tested stilbenoids were evaluated for their ability to attenuate the LPS-stimulated activation of NF-κB/AP-1. Most of the tested substances reduced the activity of NF-κB/AP-1 and later attenuated the expression of TNF-α. The effects of selected stilbenoids were further investigated on inflammatory signaling pathways. Non-prenylated stilbenoids regulated attenuation of NF-ĸB/AP-1 activity upstream by inhibiting the phosphorylation of MAPKs. A docking study used to in silico analyze the tested compounds confirmed their interaction with NF-ĸB, COX-2 and 5-LOX.
References provided by Crossref.org
Investigation of Anti-Inflammatory Potential of N-Arylcinnamamide Derivatives
Antioxidant Activity of Selected Stilbenoid Derivatives in a Cellular Model System
Metabolism of Stilbenoids by Human Faecal Microbiota
