Carbosilane dendrimers with phosphonium terminal groups are low toxic non-viral transfection vectors for siRNA cell delivery
Jazyk angličtina Země Nizozemsko Médium print-electronic
Typ dokumentu časopisecké články
PubMed
30877030
DOI
10.1016/j.ijpharm.2019.03.018
PII: S0378-5173(19)30196-6
Knihovny.cz E-zdroje
- Klíčová slova
- Gene therapy, Molecular dynamics, Non-viral vectors, Phosphonium carbosilane dendrimers, Small interfering RNA, Transfection,
- MeSH
- buněčné linie MeSH
- Cricetulus MeSH
- dánio pruhované MeSH
- dendrimery aplikace a dávkování toxicita MeSH
- embryo nesavčí MeSH
- LD50 MeSH
- malá interferující RNA aplikace a dávkování MeSH
- organofosforové sloučeniny aplikace a dávkování toxicita MeSH
- silany aplikace a dávkování toxicita MeSH
- transfekce metody MeSH
- umlčování genů MeSH
- zvířata MeSH
- Check Tag
- zvířata MeSH
- Publikační typ
- časopisecké články MeSH
- Názvy látek
- carbosilane MeSH Prohlížeč
- dendrimery MeSH
- malá interferující RNA MeSH
- organofosforové sloučeniny MeSH
- silany MeSH
Non-viral gene delivery vectors studied in the gene therapy applications are often designed with the cationic nitrogen containing groups necessary for binding and cell release of nucleic acids. Disadvantage is a relatively high toxicity which restricts the in vivo use of such nanoparticles. Here we show, that the 3rd generation carbosilane dendrimers possessing (trimethyl)phosphonium (PMe3) groups on their periphery were able to effectively deliver the functional siRNA into the cells (B14, Cricetulus griseus), release it into the cytosol and finally to achieve up to 40% gene silencing of targeted gene (glyceraldehyde-3-phosphate dehydrogenase (GAPDH)) with the comparable or, in some cases, even better effectivity as their ammonium counterparts. Moreover, such cationic dendrimers show relatively low in vivo toxicity as compared to their ammonium analogues when analyzed by standard fish embryo test (FET) on Danio rerio in vivo model, with LD50 = 6.26 µM after 48 h of incubation. This is more than 10-fold improvement as compared to published values for various other types of cationic dendrimers. We discuss the potential of further increase of the transfection efficiency, endosomal escape and decrease of toxicity of such non-viral vectors, based on the systematic screening of different types of substituents on central phosphonium atom.
Faculty of Science J E Purkyně University in Ústí nad Labem 40096 Ústí nad Labem Czech Republic
Leibniz Institute of Polymer Research Dresden Hohe Straße 6 D 01069 Dresden Germany
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