• This record comes from PubMed

Versatile Bioconjugation Strategies of PEG-Modified Upconversion Nanoparticles for Bioanalytical Applications

. 2020 Nov 09 ; 21 (11) : 4502-4513. [epub] 20200529

Language English Country United States Media print-electronic

Document type Journal Article, Research Support, Non-U.S. Gov't

Lanthanide-doped upconversion nanoparticles (UCNPs) display highly beneficial photophysical features for background-free bioimaging and bioanalysis; however, they are instable in high ionic strength buffers, have no functional groups, and are nonspecifically interacting. Here, we have prepared NIR-excitable UCNPs that are long-term colloidally stable in buffered media and possess functional groups. Heterobifunctional poly(ethylene glycol) (PEG) linkers bearing neridronate and alkyne or maleimide were attached to UCNPs via a ligand exchange. Streptavidin (SA)-conjugates were prepared by click reaction of UCNP@PEG-alkyne and SA-azide. Antihuman serum albumin pAbF antibody was modified with azide groups and conjugated to UCNP@PEG-alkyne via click reaction; alternatively, the antibody, after mild reduction of its disulfide bonds, was conjugated to UCNP@PEG-maleimide. We employed these nanoconjugates as labels for an upconversion-linked immunosorbent assay. SA-based labels achieved the lowest LOD of 0.17 ng/mL for the target albumin, which was superior compared to a fluorescence immunoassay (LOD 0.59 ng/mL) or an enzyme-linked immunoassay (LOD 0.56 ng/mL).

References provided by Crossref.org

Newest 20 citations...

See more in
Medvik | PubMed

Bright photon upconversion in LiYbF4:Tm3+@LiYF4 nanoparticles and their application for singlet oxygen generation and in immunoassay for SARS-CoV-2 nucleoprotein

. 2023 Nov ; 649 () : 49-57. [epub] 20230611

Cytotoxicity Evaluation of Photosensitizer-Conjugated Hexagonal Upconverting Nanoparticles

. 2023 May 03 ; 13 (9) : . [epub] 20230503

Rose Bengal-Modified Upconverting Nanoparticles: Synthesis, Characterization, and Biological Evaluation

. 2022 Sep 05 ; 12 (9) : . [epub] 20220905

Bioconjugates of photon-upconversion nanoparticles for cancer biomarker detection and imaging

. 2022 Apr ; 17 (4) : 1028-1072. [epub] 20220218

Poly(N,N-dimethylacrylamide)-coated upconverting NaYF4:Yb,Er@NaYF4:Nd core-shell nanoparticles for fluorescent labeling of carcinoma cells

. 2021 Nov 01 ; 11 (1) : 21373. [epub] 20211101

Cationic Polymer-Coated Magnetic Nanoparticles with Antibacterial Properties: Synthesis and In Vitro Characterization

. 2021 Sep 06 ; 10 (9) : . [epub] 20210906

PEG-Neridronate-Modified NaYF4:Gd3+,Yb3+,Tm3+/NaGdF4 Core-Shell Upconverting Nanoparticles for Bimodal Magnetic Resonance/Optical Luminescence Imaging

. 2021 Jun 08 ; 6 (22) : 14420-14429. [epub] 20210517

Find record

Citation metrics

Loading data ...

Archiving options

Loading data ...