Drought stress impacts the quality and yield of Pisum sativum. Here, we show how short periods of limited water availability during the vegetative stage of pea alters phloem sap content and how these changes are connected to strategies used by plants to cope with water deficit. We have investigated the metabolic content of phloem sap exudates and explored how this reflects P. sativum physiological and developmental responses to drought. Our data show that drought is accompanied by phloem-mediated redirection of the components that are necessary for cellular respiration and the proper maintenance of carbon/nitrogen balance during stress. The metabolic content of phloem sap reveals a shift from anabolic to catabolic processes as well as the developmental plasticity of P. sativum plants subjected to drought. Our study underlines the importance of phloem-mediated transport for plant adaptation to unfavourable environmental conditions. We also show that phloem exudate analysis can be used as a useful proxy to study stress responses in plants. We propose that the decrease in oleic acid content within phloem sap could be considered as a potential marker of early signalling events mediating drought response.

Department of Chemical Biology and Genetics Centre of the Region Haná for Biotechnological and Agricultural Research Faculty of Science Palacký University Olomouc Czech Republic

Department of Plant Biochemistry Institute of Biochemistry and Biophysics Polish Academy of Sciences ul Pawińskiego 5a Warsaw 02 106 Poland

Institute of Bioorganic Chemistry Polish Academy of Sciences Noskowskiego 12 14 Poznan 61 704 Poland

Integrative Plant Biology Team Institute of Plant Genetics Polish Academy of Sciences ul Strzeszyńska 34 Poznań 60 479 Poland

Laboratory for Integrated Molecular Plant Physiology Research Department of Biology University of Antwerp Groenenborgerlaan 171 Antwerpen 2020 Belgium

Plant Physiology Team Institute of Plant Genetics Polish Academy of Sciences ul Strzeszyńska 34 Poznań 60 479 Poland

ZMBP Center for Plant Molecular Biology University of Tübingen Tübingen Germany

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