Advanced glycosidases as ingenious biosynthetic instruments
Jazyk angličtina Země Anglie, Velká Británie Médium print-electronic
Typ dokumentu časopisecké články, práce podpořená grantem, přehledy
PubMed
33781890
DOI
10.1016/j.biotechadv.2021.107733
PII: S0734-9750(21)00039-2
Knihovny.cz E-zdroje
- Klíčová slova
- Chitinase, Enzyme engineering, Glycosidase, Glycosynthase, Oligosaccharide, Thioglycoligase, Transglycosylation, α-l-Fucosidase, β-Galactosidase, β-N-Acetylhexosaminidase,
- MeSH
- glykosidhydrolasy * genetika metabolismus MeSH
- glykosylace MeSH
- hydrolýza MeSH
- lidé MeSH
- oligosacharidy * MeSH
- substrátová specifita MeSH
- Check Tag
- lidé MeSH
- Publikační typ
- časopisecké články MeSH
- práce podpořená grantem MeSH
- přehledy MeSH
- Názvy látek
- glykosidhydrolasy * MeSH
- oligosacharidy * MeSH
Until recently, glycosidases, naturally hydrolyzing carbohydrate-active enzymes, have found few synthetic applications in industry, being primarily used for cleaving unwanted carbohydrates. With the establishment of glycosynthase and transglycosidase technology by genetic engineering, the view of glycosidases as industrial biotechnology tools has started to change. Their easy production, affordability, robustness, and substrate versatility, added to the possibility of controlling undesired side hydrolysis by enzyme engineering, have made glycosidases competitive synthetic tools. Current promising applications of engineered glycosidases include the production of well-defined chitooligomers, precious galactooligosaccharides or specialty chemicals such as glycosylated flavonoids. Other synthetic pathways leading to human milk oligosaccharides or remodeled antibodies are on the horizon. This work provides an overview of the synthetic achievements to date for glycosidases, emphasizing the latest trends and outlining possible developments in the field.
Citace poskytuje Crossref.org
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Flavonoids as Aglycones in Retaining Glycosidase-Catalyzed Reactions: Prospects for Green Chemistry
Engineered Glycosidases for the Synthesis of Analogs of Human Milk Oligosaccharides
Cross-Linking Effects Dictate the Preference of Galectins to Bind LacNAc-Decorated HPMA Copolymers
