Advanced glycosidases as ingenious biosynthetic instruments
Language English Country England, Great Britain Media print-electronic
Document type Journal Article, Research Support, Non-U.S. Gov't, Review
PubMed
33781890
DOI
10.1016/j.biotechadv.2021.107733
PII: S0734-9750(21)00039-2
Knihovny.cz E-resources
- Keywords
- Chitinase, Enzyme engineering, Glycosidase, Glycosynthase, Oligosaccharide, Thioglycoligase, Transglycosylation, α-l-Fucosidase, β-Galactosidase, β-N-Acetylhexosaminidase,
- MeSH
- Glycoside Hydrolases * genetics metabolism MeSH
- Glycosylation MeSH
- Hydrolysis MeSH
- Humans MeSH
- Oligosaccharides * MeSH
- Substrate Specificity MeSH
- Check Tag
- Humans MeSH
- Publication type
- Journal Article MeSH
- Research Support, Non-U.S. Gov't MeSH
- Review MeSH
- Names of Substances
- Glycoside Hydrolases * MeSH
- Oligosaccharides * MeSH
Until recently, glycosidases, naturally hydrolyzing carbohydrate-active enzymes, have found few synthetic applications in industry, being primarily used for cleaving unwanted carbohydrates. With the establishment of glycosynthase and transglycosidase technology by genetic engineering, the view of glycosidases as industrial biotechnology tools has started to change. Their easy production, affordability, robustness, and substrate versatility, added to the possibility of controlling undesired side hydrolysis by enzyme engineering, have made glycosidases competitive synthetic tools. Current promising applications of engineered glycosidases include the production of well-defined chitooligomers, precious galactooligosaccharides or specialty chemicals such as glycosylated flavonoids. Other synthetic pathways leading to human milk oligosaccharides or remodeled antibodies are on the horizon. This work provides an overview of the synthetic achievements to date for glycosidases, emphasizing the latest trends and outlining possible developments in the field.
References provided by Crossref.org
Three-Step Enzymatic Remodeling of Chitin into Bioactive Chitooligomers
Flavonoids as Aglycones in Retaining Glycosidase-Catalyzed Reactions: Prospects for Green Chemistry
Engineered Glycosidases for the Synthesis of Analogs of Human Milk Oligosaccharides
Cross-Linking Effects Dictate the Preference of Galectins to Bind LacNAc-Decorated HPMA Copolymers
