Interactions among endosymbiotic bacteria inside their eukaryotic hosts are poorly understood, particularly in mites. The mite Tyrophagus putrescentiae is a common, medically important generalist species that has many intracellular and gut bacterial symbionts. In the experiments, we examined bacterial abundances and composition in mite populations obtained by controlled mixing of stock mite populations that differed in the presence/absence of the major intracellular bacteria Wolbachia and Cardinium. Changes in microbial communities were characterized using 16S ribosomal RNA high-throughput sequencing (pooled mite individuals) and quantitative PCR for key microbial taxa (individual mites). Mite fitness was estimated as a parameter of population growth. We detected that in mixed mite populations, Cardinium and Wolbachia can co-occur in the same mite individual. The presence of Cardinium was negatively correlated with the presence of Wolbachia and Bartonella, while the Bartonella and Wolbachia were positively correlated in individual level samples. Since mixed populations had lower abundances of Wolbachia, while the abundance of Cardinium did not change, we suggest that the presence of Cardinium inhibits the growth of Wolbachia. The mixed mite populations had lower population growth than parental populations. The possible effect of symbionts on the fitness of mixed population is discussed.

Crop Research Institute Drnovska 507 73 CZ 16106 Prague 6 Ruzyne Czechia

Department of Botany and Zoology Faculty of Science Masaryk University Kotlarska 2 CZ 611 37 Brno Czechia

Faculty of Agrobiology Food and Natural Resources Czech University of Life Sciences Prague Kamycka 129 CZ 165 00 Prague 6 Suchdol Czechia

Genomics and Microbiome Core Facility Rush University Chicago IL 60612 USA

Institute of Biology University of Tyumen Pirogova 3 625043 Tyumen Russia

School of Natural Sciences Bangor University Bangor LL57 2 UW UK

References provided by Crossref.org

Analysis of the tripartite interactions between two bacterial symbionts, a novel Solitalea-like bacterium (Bacteroidota) and Cardinium, and the stored product mite Tyrophagus putrescentiae based on gene expression data

Cardinium disrupts Wolbachia-host dynamics in the domestic mite Tyrophagus putrescentiae: evidence from manipulative experiments

A novel Erwiniaceae gut symbiont modulates gene expression of the intracellular bacterium Cardinium in the stored product mite Tyrophagus putrescentiae

A novel Bartonella-like bacterium forms an interdependent mutualistic symbiosis with its host, the stored-product mite Tyrophagus putrescentiae

Mixta mediterraneensis as a novel and abundant gut symbiont of the allergen-producing domestic mite Blomia tropicalis

The Effect of Residual Pesticide Application on Microbiomes of the Storage Mite Tyrophagus putrescentiae

Endogenous Plasmids and Chromosomal Genome Reduction in the Cardinium Endosymbiont of Dermatophagoides farinae