Over the last two decades, increased interest of scientists to study bone marrow adiposity (BMA) in relation to bone and adipose tissue physiology has expanded the number of publications using different sources of bone marrow adipose tissue (BMAT). However, each source of BMAT has its limitations in the number of downstream analyses for which it can be used. Based on this increased scientific demand, the International Bone Marrow Adiposity Society (BMAS) established a Biobanking Working Group to identify the challenges of biobanking for human BMA-related samples and to develop guidelines to advance establishment of biobanks for BMA research. BMA is a young, growing field with increased interest among many diverse scientific communities. These bring new perspectives and important biological questions on how to improve and build an international community with biobank databases that can be used and shared all over the world. However, to create internationally accessible biobanks, several practical and legislative issues must be addressed to create a general ethical protocol used in all institutes, to allow for exchange of biological material internationally. In this position paper, the BMAS Biobanking Working Group describes similarities and differences of patient information (PIF) and consent forms from different institutes and addresses a possibility to create uniform documents for BMA biobanking purposes. Further, based on discussion among Working Group members, we report an overview of the current isolation protocols for human bone marrow adipocytes (BMAds) and bone marrow stromal cells (BMSCs, formerly mesenchymal), highlighting the specific points crucial for effective isolation. Although we remain far from a unified BMAd isolation protocol and PIF, we have summarized all of these important aspects, which are needed to build a BMA biobank. In conclusion, we believe that harmonizing isolation protocols and PIF globally will help to build international collaborations and improve the quality and interpretation of BMA research outcomes.

British Heart Foundation Centre for Cardiovascular Science The Queen's Medical Research Institute University of Edinburgh Edinburgh United Kingdom

Center for Molecular Medicine Maine Medical Center Research Institute Scarborough ME United States

Clinical Cell Biology Department of Pathology Odense University Hospital Odense Denmark

Clinical Cell Biology Pathology Research Unit Department of Clinical Research University of Southern Denmark Odense Denmark

Department of Biochemistry and Molecular Cell Biology University Medical Center Hamburg Eppendorf Hamburg Germany

Department of Biomedical Sciences Faculty of Biology and Medicine Université de Lausanne Lausanne Switzerland

Department of Forensic Medicine Aarhus University Aarhus Denmark

Department of Molecular Medicine University of Southern Denmark Odense Denmark

Department of Orthopedics University Medical Center Hamburg Eppendorf Hamburg Germany

Department of Pharmacology Wayne State University School of Medicine and Karmanos Cancer Institute Detroit MI United States

Equipe labellisée Ligue contre le cancer Toulouse France

Graduate School for Biomedical Science Tufts University Boston MA United States

Hematology Service Departments of Oncology and Laboratory Medicine Lausanne University Hospital Université de Lausanne Lausanne Switzerland

Institute of Biomedicine University of Turku Turku Finland

Institute of Pharmacology and Structural Biology Université de Toulouse CNRS UMR 5089 Toulouse France

Laboratory for Calcium and Bone Metabolism Department of Internal Medicine Erasmus University Medical Center Rotterdam Netherlands

Marrow Adiposity and Bone Lab MABLab ULR4490 Univ Littoral Côte d'Opale Boulogne sur Mer Univ Lille CHU Lille Lille France

Molecular Physiology of Bone Institute of Physiology of the Czech Academy of Sciences Prague Czechia

School of Life Sciences École Polytechnique Fédérale de Lausanne Lausanne Switzerland

Swiss Institute of Bioinformatics Lausanne Switzerland

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