Bacterial microbiota play an important role in the fitness of arthropods, but the bacterial microflora in the parasitic mite Dermanyssus gallinae is only partially explored; there are gaps in our understanding of the microbiota localization and in our knowledge of microbial community assembly. In this work, we have visualized, quantified the abundance, and determined the diversity of bacterial occupancy, not only across developmental stages of D. gallinae, but also in the midgut of micro-dissected female D. gallinae mites. We explored community assembly and the presence of keystone taxa, as well as predicted metabolic functions in the microbiome of the mite. The diversity of the microbiota and the complexity of co-occurrence networks decreased with the progression of the life cycle. However, several bacterial taxa were present in all samples examined, indicating a core symbiotic consortium of bacteria. The relatively higher bacterial abundance in adult females, specifically in their midguts, implicates a function linked to the biology of D. gallinae mites. If such an association proves to be important, the bacterial microflora qualifies itself as an acaricidal or vaccine target against this troublesome pest.

ANSES INRAE Ecole Nationale Vétérinaire d'Alfort UMR BIPAR Laboratoire de Santé Animale Maisons Alfort France

EA 7310 Laboratoire de Virologie Université de Corse Corte France

INRAE UR 0045 Laboratoire de Recherches Sur Le Développement de L'Elevage 20250 Corte France

Institute of Parasitology Biology Centre Czech Academy of Sciences 37005 Ceske Budejovice Czech Republic

School of Environmental Sciences University of Guelph Guelph ON N1G 2W1 Canada

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One health approach to study human health risks associated with Dermanyssus gallinae mites

Significant role of symbiotic bacteria in the blood digestion and reproduction of Dermanyssus gallinae mites