Caspase-9 is traditionally considered the initiator caspase of the intrinsic apoptotic pathway. In the past decade, however, other functions beyond initiation/execution of cell death have been described including cell type-dependent regulation of proliferation, differentiation/maturation, mitochondrial, and endosomal/lysosomal homeostasis. As previous studies revealed nonapoptotic functions of caspases in osteogenesis and bone homeostasis, this study was performed to identify proteins and pathways deregulated by knockout of caspase-9 in mouse MC3T3-E1 osteoblasts. Data-independent acquisition-parallel accumulation serial fragmentation (diaPASEF) proteomics was used to compare protein profiles of control and caspase-9 knockout cells. A total of 7669 protein groups were quantified, and 283 upregulated/141 downregulated protein groups were associated with the caspase-9 knockout phenotype. The deregulated proteins were mainly enriched for those associated with cell migration and motility and DNA replication/repair. Altered migration was confirmed in MC3T3-E1 cells with the genetic and pharmacological inhibition of caspase-9. ABHD2, an established regulator of cell migration, was identified as a possible substrate of caspase-9. We conclude that caspase-9 acts as a modulator of osteoblastic MC3T3-E1 cell migration and, therefore, may be involved in bone remodeling and fracture repair.

Department of Biochemistry Faculty of Science Masaryk University Brno 625 00 Czech Republic

Department of Experimental Biology Faculty of Science Masaryk University Brno 625 00 Czech Republic

Department of Physiology Faculty of Veterinary Medicine University of Veterinary Sciences Brno 612 42 Czech Republic

International Clinical Research Center St Anne's University Hospital Brno 602 00 Czech Republic

Laboratory of Odontogenesis and Osteogenesis Institute of Animal Physiology and Genetics Czech Academy of Sciences Brno 602 00 Czech Republic

Proteomics Core Facility Central European Institute for Technology Masaryk University Brno 625 00 Czech Republic

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