Multifunctional polymers are interesting substances for the formulation of drug molecules that cannot be administered in their pure form due to their pharmacokinetic profiles or side effects. Polymer-drug formulations can enhance pharmacological properties or create tissue specificity by encapsulating the drug into nanocontainers, or stabilizing nanoparticles for drug transport. We present the synthesis of multifunctional poly(2-ethyl-2-oxazoline-co-2-glyco-2-oxazoline)s containing two reactive end groups, and an additional hydrophobic anchor at one end of the molecule. These polymers were successfully used to stabilize (solid) lipid nanoparticles ((S)LNP) consisting of tetradecan-1-ol and cholesterol with their hydrophobic anchor. While the pure polymers interacted with GLUT1-expressing cell lines mainly based on their physicochemical properties, especially via interactions of the hydrophobic anchor with membranous compartments of the cells, LNP-cell interactions hinted toward an influence of the glucosylation on particle-cell interactions. The presented LNP are therefore promising systems for the delivery of drugs into GLUT1-expressing cell lines.

Institute of Biochemistry and Experimental Oncology 1st Faculty of Medicine U Nemocnice 5 Praha 2 128 53 Czech Republic

Institute of Macromolecular Chemistry CAS Heyrovského nám 2 Praha 6 162 06 Czech Republic

Institute of Organic Chemistry and Biochemistry CAS Flemingovo nám 2 Praha 6 166 10 Czech Republic

Institute of Scientific Instruments CAS Královopolská 147 Brno 612 00 Czech Republic

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