Francisella tularensis is a zoonotic, gram-negative bacterium that causes tularemia in humans. Depending on its subspecies and the route of transmission, mild to lethal courses have been reported. F. tularensis subsp. holarctica is the only subspecies found in Europe and affects a plenitude of vertebrates including lagomorphs and rodents. Population outbreaks of certain rodent species are likely to be involved in the transmission of this pathogen. This molecular survey aims to evaluate the presence of F. tularensis in small mammals from three Central European countries. Using a real-time polymerase chain reaction, F. tularensis DNA was detected in common voles (Microtus arvalis) from Switzerland and in field voles (Microtus agrestis) and a bank vole (Myodes glareolus) from Germany, but not in any other small mammal species. All common voles from the Czech Republic were negative for F. tularensis DNA. The prevalence in the three vole species varied between 1.3% and 3.0%. In conclusion, Francisella tularensis DNA was detected in three vole species in two of three countries investigated. The observed low prevalence raises questions on the role of voles for the transmission of Francisella tularensis in Central Europe.

• MeSH
• Arvicolinae mikrobiologie   MeSH
• DNA bakterií genetika   MeSH
• epidemický výskyt choroby veterinární   MeSH
• Francisella tularensis genetika   izolace a purifikace   MeSH
• kvantitativní polymerázová řetězová reakce veterinární   MeSH
• lidé MeSH
• tularemie epidemiologie   mikrobiologie   veterinární   MeSH
• zvířata MeSH
• Check Tag
• lidé MeSH
• zvířata MeSH
• Publikační typ
• časopisecké články MeSH
• Geografické názvy
• Evropa MeSH

BACKGROUND: While the 'no-tillage' management system generally improves soil properties and helps to control arthropod pests, it may also intensify crop infestation by the common vole (Microtus arvalis Pallas). In this study, we evaluated the impact of soil management (no-tillage or tillage), crop and previous crop (winter wheat or winter rape), and season (spring or autumn) on common vole density using data from the Common Vole Monitoring Programme undertaken by the Plant Protection Service of the Czech Republic between 2000 and 2009. RESULTS: Models predicted low mean values of vole infestation across management types, crops, and seasons. The untilled fields hosted significantly more voles than the tilled fields in spring but not in autumn. More common voles were found in winter rape than in winter wheat during both seasons. CONCLUSION: Recent studies suggest that no-tillage management is more profitable than tillage management as a result of its positive impact on soil properties and pest control. During periods of high vole infestation, however, tillage may constitute an alternative strategy for reducing yield losses. © 2017 Society of Chemical Industry.

• MeSH
• Arvicolinae fyziologie   MeSH
• biologické modely MeSH
• Brassica napus růst a vývoj   MeSH
• hustota populace MeSH
• pěstování plodin metody   MeSH
• pšenice růst a vývoj   MeSH
• roční období MeSH
• zvířata MeSH
• Check Tag
• zvířata MeSH
• Publikační typ
• časopisecké články MeSH
• Geografické názvy
• Česká republika MeSH