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Faculty of Biomedical Engineering Cze... 1 Faculty of Biomedical Engineering Cze... 1 Faculty of Biomedical Engineering Cze... 1 Faculty of Biomedical Engineering Cze... 1 Generi Biotech Ltd Machkova 587 500 1... 1 Institute of Organic Chemistry and Bi... 1 Nuclear Physics Institute AS CR v v i... 1
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Faculty of Biomedical Engineering Cze... 1 Faculty of Biomedical Engineering Cze... 1 Faculty of Biomedical Engineering Cze... 1 Faculty of Biomedical Engineering Cze... 1 Generi Biotech Ltd Machkova 587 500 1... 1 Institute of Organic Chemistry and Bi... 1 Nuclear Physics Institute AS CR v v i... 1
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- Petrakova, V
- Benson, V
- Buncek, M
- Fiserova, A
- Ledvina, M
- Stursa, J
- Cigler, P
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Nesladek, M
Autor Nesladek, M Faculty of Biomedical Engineering, Czech Technical University in Prague, Sitna sq. 3105, 272 01 Kladno, Czech Republic and IMEC Division IMOMEC, Hasselt University, Wetenschapspark 1, B-3590, Diepenbeek, Belgium and Institute for Materials Research, Hasselt University, Wetenschapspark 1, B-3590 Diepenbeek, Belgium. milos.nesladek@uhasselt.be
Digitální knihovna NLK
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Zdroj
PubMed
27240633
DOI
10.1039/c6nr00610h
Knihovny.cz E-zdroje
Efficient delivery of stabilized nucleic acids (NAs) into cells and release of the NA payload are crucial points in the transfection process. Here we report on the fabrication of a nanoscopic cellular delivery carrier that is additionally combined with a label-free intracellular sensor device, based on biocompatible fluorescent nanodiamond particles. The sensing function is engineered into nanodiamonds by using nitrogen-vacancy color centers, providing stable non-blinking luminescence. The device is used for monitoring NA transfection and the payload release in cells. The unpacking of NAs from a poly(ethyleneimine)-terminated nanodiamond surface is monitored using the color shift of nitrogen-vacancy centers in the diamond, which serve as a nanoscopic electric charge sensor. The proposed device innovates the strategies for NA imaging and delivery, by providing detection of the intracellular release of non-labeled NAs without affecting cellular processing of the NAs. Our system highlights the potential of nanodiamonds to act not merely as labels but also as non-toxic and non-photobleachable fluorescent biosensors reporting complex molecular events.
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- buňky HT-29 MeSH
- DNA * MeSH
- lidé MeSH
- luminiscence MeSH
- myši inbrední DBA MeSH
- nanodiamanty * MeSH
- transfekce * MeSH
- zvířata MeSH
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- lidé MeSH
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