Amine-coated biodegradable materials based on synthetic polymers have a great potential for tissue remodeling and regeneration because of their excellent processability and bioactivity. In the present study, we have investigated the influence of various chemical compositions of amine plasma polymer (PP) coatings and the influence of the substrate morphology, represented by polystyrene culture dishes and polycaprolactone nanofibers (PCL NFs), on the behavior of vascular smooth muscle cells (VSMCs). Although all amine-PP coatings improved the initial adhesion of VSMCs, 7-day long cultivation revealed a clear preference for the coating containing about 15 at.% of nitrogen (CPA-33). The CPA-33 coating demonstrated the ideal combination of good water stability, a sufficient amine group content, and favorable surface wettability and morphology. The nanostructured morphology of amine-PP-coated PCL NFs successfully slowed the proliferation rate of VSMCs, which is essential in preventing restenosis of vascular replacements in vivo. At the same time, CPA-33-coated PCL NFs supported the continuous proliferation of VSMCs during 7-day long cultivation, with no significant increase in cytokine secretion by RAW 264.7 macrophages. The CPA-33 coating deposited on biodegradable PCL NFs therefore seems to be a promising material for manufacturing small-diameter vascular grafts, which are still lacking on the current market.

• MeSH
• aminy škodlivé účinky   chemie   imunologie   farmakologie   MeSH
• biokompatibilní potahované materiály škodlivé účinky   chemie   farmakologie   MeSH
• buněčná adheze účinky léků   imunologie   MeSH
• fotoelektronová spektroskopie MeSH
• krevní plazma chemie   imunologie   MeSH
• krysa rodu rattus MeSH
• kultivované buňky MeSH
• makrofágy účinky léků   metabolismus   MeSH
• myocyty hladké svaloviny účinky léků   metabolismus   MeSH
• myši MeSH
• nanovlákna škodlivé účinky   chemie   MeSH
• polyestery chemie   MeSH
• polymery škodlivé účinky   chemie   farmakologie   MeSH
• povrchové vlastnosti účinky léků   MeSH
• proliferace buněk účinky léků   MeSH
• RAW 264.7 buňky MeSH
• svaly hladké cévní cytologie   účinky léků   růst a vývoj   MeSH
• tkáňové podpůrné struktury škodlivé účinky   chemie   MeSH
• zvířata MeSH
• Check Tag
• krysa rodu rattus MeSH
• myši MeSH
• zvířata MeSH
• Publikační typ
• časopisecké články MeSH