Amine-coated biodegradable materials based on synthetic polymers have a great potential for tissue remodeling and regeneration because of their excellent processability and bioactivity. In the present study, we have investigated the influence of various chemical compositions of amine plasma polymer (PP) coatings and the influence of the substrate morphology, represented by polystyrene culture dishes and polycaprolactone nanofibers (PCL NFs), on the behavior of vascular smooth muscle cells (VSMCs). Although all amine-PP coatings improved the initial adhesion of VSMCs, 7-day long cultivation revealed a clear preference for the coating containing about 15 at.% of nitrogen (CPA-33). The CPA-33 coating demonstrated the ideal combination of good water stability, a sufficient amine group content, and favorable surface wettability and morphology. The nanostructured morphology of amine-PP-coated PCL NFs successfully slowed the proliferation rate of VSMCs, which is essential in preventing restenosis of vascular replacements in vivo. At the same time, CPA-33-coated PCL NFs supported the continuous proliferation of VSMCs during 7-day long cultivation, with no significant increase in cytokine secretion by RAW 264.7 macrophages. The CPA-33 coating deposited on biodegradable PCL NFs therefore seems to be a promising material for manufacturing small-diameter vascular grafts, which are still lacking on the current market.
- MeSH
- aminy škodlivé účinky chemie imunologie farmakologie MeSH
- biokompatibilní potahované materiály škodlivé účinky chemie farmakologie MeSH
- buněčná adheze účinky léků imunologie MeSH
- fotoelektronová spektroskopie MeSH
- krevní plazma chemie imunologie MeSH
- krysa rodu rattus MeSH
- kultivované buňky MeSH
- makrofágy účinky léků metabolismus MeSH
- myocyty hladké svaloviny účinky léků metabolismus MeSH
- myši MeSH
- nanovlákna škodlivé účinky chemie MeSH
- polyestery chemie MeSH
- polymery škodlivé účinky chemie farmakologie MeSH
- povrchové vlastnosti účinky léků MeSH
- proliferace buněk účinky léků MeSH
- RAW 264.7 buňky MeSH
- svaly hladké cévní cytologie účinky léků růst a vývoj MeSH
- tkáňové podpůrné struktury škodlivé účinky chemie MeSH
- zvířata MeSH
- Check Tag
- krysa rodu rattus MeSH
- myši MeSH
- zvířata MeSH
- Publikační typ
- časopisecké články MeSH
The study monitored in vitro early response of connective tissue cells and immunocompetent cells to enosseal implant materials coated by different blood components (serum, activated plasma, and plasma/platelets) to evaluate human osteoblast proliferation and synthetic activity and inflammatory response presented as a cytokine profile of peripheral blood mononuclear cells (PBMCs) under conditions imitating the situation upon implantation. The cells were cultivated on coated Ti-plasma-sprayed (Ti-PS), Ti-etched (Ti-Etch), Ti-hydroxyapatite (Ti-HA), and ZrO2 surfaces. The plasma/platelets coating supported osteoblast proliferation only on osteoconductive Ti-HA and Ti-Etch whereas activated plasma enhanced proliferation on all surfaces. Differentiation (BAP) and IL-8 production remained unchanged or decreased irrespective of the coating and surface; only the serum and plasma/platelets-coated ZrO2 exhibited higher BAP and IL-8 expression. RANKL production increased on serum and activated plasma coatings. PBMCs produced especially cytokines playing role in inflammatory phase of wound healing, that is, IL-6, GRO-α, GRO, ENA-78, IL-8, GM-CSF, EGF, and MCP-1. Cytokine profiles were comparable for all tested surfaces; only ENA-78, IL-8, GM-CSF, and MCP-1 expression depended on materials and coatings. The activated plasma coating led to uniformed surfaces and represented a favorable treatment especially for bioinert Ti-PS and ZrO2 whereas all coatings had no distinctive effect on bioactive Ti-HA and Ti-Etch.
- MeSH
- biokompatibilní potahované materiály škodlivé účinky chemie MeSH
- buněčné linie MeSH
- chemokin CCL2 metabolismus MeSH
- chemokin CXCL1 metabolismus MeSH
- chemokin CXCL5 metabolismus MeSH
- cytokiny metabolismus MeSH
- epidermální růstový faktor metabolismus MeSH
- faktor stimulující granulocyto-makrofágové kolonie metabolismus MeSH
- hojení ran účinky léků MeSH
- interleukin-6 metabolismus MeSH
- interleukin-8 metabolismus MeSH
- leukocyty mononukleární účinky léků metabolismus MeSH
- lidé MeSH
- osteoblasty účinky léků metabolismus MeSH
- proliferace buněk účinky léků MeSH
- titan škodlivé účinky chemie MeSH
- zánět metabolismus MeSH
- Check Tag
- lidé MeSH
- Publikační typ
- časopisecké články MeSH
