Despite novel treatment strategies, multiple myeloma (MM) remains an incurable disease with low immunogenicity and multiple immune defects. We developed an ex vivo strategy for inducing myeloma-specific cytotoxic T lymphocytes (CTLs) and demonstrate the possibility of identification and long-term in vivo monitoring of individual myeloma-specific T-cell clones using the most sensitive clonotypic assay that is able to detect low frequencies of T-cell clones (1 clonotypic cell in 10(6) cells). Ten patients with MM were examined for the presence of tumour-reactive T cells using dendritic cells loaded with autologous tumour cells. All patients had detectable myeloma-reactive T cells in vitro. Expanded myeloma-reactive T cells demonstrated specific cytotoxic effects against autologous tumour cells in vitro (median 39.6% at an effector:target ratio of 40:1). The clonality of myeloma-specific T cells was studied with a clonotypic assay, which demonstrated both oligoclonal and monoclonal populations of myeloma-specific T cells. CD8(+) CTLs were the most immunodominant myeloma-specific T-cell clones and clinical responses were closely associated with the in vivo expansion and long-term persistence of individual CD8(+) T-cell clones, usually at very low frequencies (10(-3)-10(-6)). We conclude that the clonotypic assay is the most sensitive tool for immunomonitoring of low-frequency T cells.

• MeSH
• aktivace lymfocytů imunologie   MeSH
• antigeny nádorové imunologie   MeSH
• buněčná diferenciace imunologie   MeSH
• buněčné klony imunologie   MeSH
• cytotoxicita imunologická imunologie   MeSH
• cytotoxické T-lymfocyty imunologie   MeSH
• dendritické buňky imunologie   MeSH
• hypervariabilní oblasti genetika   imunologie   MeSH
• imunodominantní epitopy imunologie   MeSH
• imunomagnetická separace metody   MeSH
• lidé středního věku MeSH
• lidé MeSH
• mnohočetný myelom imunologie   terapie   MeSH
• molekulární sekvence - údaje MeSH
• monitorování imunologické metody   MeSH
• nádorové buňky kultivované MeSH
• následné studie MeSH
• prezentace antigenu imunologie   MeSH
• sekvence aminokyselin MeSH
• senioři MeSH
• Check Tag
• lidé středního věku MeSH
• lidé MeSH
• mužské pohlaví MeSH
• senioři MeSH
• ženské pohlaví MeSH
• Publikační typ
• časopisecké články MeSH
• práce podpořená grantem MeSH