The retention behavior of the unmodified phosphodiester oligonucleotide sequence isomers was investigated on two different anion exchange columns: Biospher GMB 1000Q (based on DEAE-modified glycidyl methacrylate) and PolyWAX LP (based on silica with a crosslinked coating of linear polyethyleneimine). There was a notable difference in retention of oligonucleotides of the same composition but differing in the position of a single base. The most pronounced difference was observed between the oligonucleotides with the variable base in the end and in the center of the sequence. The use of either acetonitrile or 2-propanol as a mobile phase organic modifier did not markedly affect the retention time patterns. Prediction of the retention times of oligonucleotides must take into account the base position as well as identity. This is the first report of such a "same composition different sequence" effect, described for the short peptides, for synthetic oligonucleotides.
Závěrečná zpráva o řešení grantu Interní grantové agentury MZ ČR
51 l. : grafy ; 32 cm
Projekt je zaměřen na studium genově cílených triplax tvořících oligonukleotidů a jejich konjugátů s DNA-destrujícími molekulami aktivovatelnými blízce infračerveným zářením v genové terapii HIV infekce. Projekt je založen na vyhledávání vhodných sekvencí pro TFO v genomu proviru HIV,návrh TFO a studium vazebných a štěpících schopnosti TFO in vitro a v buněčné kultuře metodami blíže popsanými v tomto projektu.; Project is focused on study of gene tergeted triplex forming oligonucleotides (TFO) and their conjugates with red-shifted wavelenghts photoactivable DNA-destructing molecules in anti-HIV gene therapy. The project is based on triplex terget sites in HIV provirus search,TFOs designing and evalution of binding and cleavage properties of TFOs in vitro and in cell culture by in project described methods.
