The authors wish to make the following corrections to this paper [...].
- Publikační typ
- tisková chyba MeSH
Fullerene derivatives with hydrophilic substituents have been shown to exhibit a range of biological activities, including antiviral ones. For a long time, the anti-HIV activity of fullerene derivatives was believed to be due to their binding into the hydrophobic pocket of HIV-1 protease, thereby blocking its activity. Recent work, however, brought new evidence of a novel, protease-independent mechanism of fullerene derivatives' action. We studied in more detail the mechanism of the anti-HIV-1 activity of N,N-dimethyl[70]fulleropyrrolidinium iodide fullerene derivatives. By using a combination of in vitro and cell-based approaches, we showed that these C70 derivatives inhibited neither HIV-1 protease nor HIV-1 maturation. Instead, our data indicate effects of fullerene C70 derivatives on viral genomic RNA packaging and HIV-1 cDNA synthesis during reverse transcription-without impairing reverse transcriptase activity though. Molecularly, this could be explained by a strong binding affinity of these fullerene derivatives to HIV-1 nucleocapsid domain, preventing its proper interaction with viral genomic RNA, thereby blocking reverse transcription and HIV-1 infectivity. Moreover, the fullerene derivatives' oxidative activity and fluorescence quenching, which could be one of the reasons for the inconsistency among reported anti-HIV-1 mechanisms, are discussed herein.
- MeSH
- fullereny metabolismus farmakologie MeSH
- genom virový účinky léků MeSH
- genové produkty gag - virus lidské imunodeficience metabolismus MeSH
- HEK293 buňky MeSH
- HIV-1 účinky léků genetika metabolismus fyziologie MeSH
- látky proti HIV metabolismus farmakologie MeSH
- lidé MeSH
- nukleokapsida - proteiny metabolismus MeSH
- reverzní transkripce MeSH
- RNA virová metabolismus MeSH
- svlékání virového obalu účinky léků MeSH
- vazba proteinů MeSH
- virion metabolismus MeSH
- zabalení virového genomu účinky léků MeSH
- Check Tag
- lidé MeSH
- Publikační typ
- časopisecké články MeSH
- práce podpořená grantem MeSH
Psyché
Vydání 1. 280 stran : ilustrace, tabulky ; 21 cm
Vysokoškolský učební text, který se zaměřuje na závislosti, jejich diagnostiku a léčbu.
- MeSH
- diferenciální diagnóza MeSH
- duševně nemocní psychologie MeSH
- duševní poruchy MeSH
- návykové chování MeSH
- psychiatrická rehabilitace MeSH
- psychologické techniky MeSH
- psychoterapie MeSH
- uživatelé drog psychologie MeSH
- Konspekt
- Psychopatologie
- Učební osnovy. Vyučovací předměty. Učebnice
- NLK Obory
- psychologie, klinická psychologie
- psychiatrie
- adiktologie
- NLK Publikační typ
- učebnice vysokých škol
Karta pacienta je psychoterapeutická pomůcka, která je aktuálně pilotována na akutním oddělení v PN Bohnice. Cílem Karty pacienta je efektivnější psychoterapeutická práce během hospitalizace na akutním psychiatrickém oddělení. V příspěvku jsou popsány její jednotlivé části a cíle, ke kterým je směřována. Karta pacienta vznikla z praktických potřeb psychiatrického oddělení a byla volně inspirována teoretickými koncepty ACT, PACT, motivačními rozhovory a prvky podpory self-efficacy. Karta pacienta je aktuálně pilotována a její účinek a význam by měl být v budoucnosti prověřen výzkumnými studiemi.
The article is focused on presentation of a new psychotherapeutic tool, which is in these days newly used and tested in acute department in Psychiatric Hospital Bohnice. This psychotherapeutic tool named Patient Card and its goal is to provide more effective psychoterapy during the short-term hospitalization. All parts of the Card and also goals of this parts are described. The Patient Card was created from the practical needs of the psychiatric deparment and was freely inspired by the theoretical concepts of ACT, PACT, motivational interviewing and parts of self-efficacy support. The Patient Card is currenty being piloted and its effectivness and significance should be verified in the future by further research studies.
Mason-Pfizer monkey virus (M-PMV), like some other betaretroviruses, encodes a G-patch domain (GPD). This glycine-rich domain, which has been predicted to be an RNA binding module, is invariably localized at the 3' end of the pro gene upstream of the pro-pol ribosomal frameshift sequence of genomic RNAs of betaretroviruses. Following two ribosomal frameshift events and the translation of viral mRNA, the GPD is present in both Gag-Pro and Gag-Pro-Pol polyproteins. During the maturation of the Gag-Pro polyprotein, the GPD transiently remains a C-terminal part of the protease (PR), from which it is then detached by PR itself. The destiny of the Gag-Pro-Pol-encoded GPD remains to be determined. The function of the GPD in the retroviral life cycle is unknown. To elucidate the role of the GPD in the M-PMV replication cycle, alanine-scanning mutational analysis of its most highly conserved residues was performed. A series of individual mutations as well as the deletion of the entire GPD had no effect on M-PMV assembly, polyprotein processing, and RNA incorporation. However, a reduction of the reverse transcriptase (RT) activity, resulting in a drop in M-PMV infectivity, was determined for all GPD mutants. Immunoprecipitation experiments suggested that the GPD is a part of RT and participates in its function. These data indicate that the M-PMV GPD functions as a part of reverse transcriptase rather than protease.
- MeSH
- buněčné linie MeSH
- lidé MeSH
- Masonův-Pfizerův opičí virus chemie enzymologie genetika MeSH
- polyproteiny chemie genetika metabolismus MeSH
- reverzní transkriptasa chemie genetika metabolismus MeSH
- terciární struktura proteinů MeSH
- virové proteiny chemie genetika metabolismus MeSH
- zvířata MeSH
- Check Tag
- lidé MeSH
- zvířata MeSH
- Publikační typ
- časopisecké články MeSH
- práce podpořená grantem MeSH
