This review defines limits of currently used techniques to assess developmental capacity of human embryos in assisted reproduction and provides an overview of techniques assessing embryo's physiology on levels of genomics, transcriptomics, proteomics and metabolomics. Basic principles of respective techniques are included. Discovered biomarkers are discussed with respect to biochemical functions and their prognostic values of embryonal development.
- Klíčová slova
- vývojový potenciál embrya,
- MeSH
- 2D gelová elektroforéza MeSH
- aneuploidie MeSH
- asistovaná reprodukce MeSH
- biologické markery analýza metabolismus MeSH
- biopsie MeSH
- embryo savčí * MeSH
- embryonální vývoj * genetika MeSH
- fertilizace in vitro MeSH
- hybridizace in situ fluorescenční metody MeSH
- lidé MeSH
- messenger RNA analýza MeSH
- metabolom MeSH
- metabolomika klasifikace metody MeSH
- polymerázová řetězová reakce metody MeSH
- preimplantační diagnóza metody MeSH
- proteom analýza MeSH
- sekvenční analýza DNA metody MeSH
- srovnávací genomová hybridizace metody MeSH
- transkriptom MeSH
- Check Tag
- lidé MeSH
- Publikační typ
- práce podpořená grantem MeSH
- přehledy MeSH
Human-assisted reproduction is increasing in importance due to the constantly rising number of couples suffering from infertility issue. A key step in in vitro fertilization is the proper assessment of embryo viability in order to select the embryo with the highest likelihood of resulting in a pregnancy. This study proposes a method based on CE with contactless conductivity detection for the determination of pyruvate and lactate in spent culture media used in human-assisted reproduction. A fused-silica capillary of 64.0 cm total length and 50 μm inner diameter was used. The inner capillary wall was modified by the coating of successive layers of the ionic polymers polybrene and dextran sulfate to reverse EOF. The BGE was composed of 10 mM MES/lithium hydroxide, pH 6.50. The sample was injected by pressure 50 mbar for 18 s, separation voltage was set to -24 kV, and capillary temperature to 15°C. The presented method requires only 2 μL of the culture medium, with LODs for pyruvate and lactate of 0.03 and 0.02 μM, respectively. The results demonstrated the method's suitability for the analysis of spent culture media to support embryo viability assessment by light microscopy, providing information about key metabolites of the energy metabolism of a developing embryo.
- MeSH
- asistovaná reprodukce * MeSH
- biologické markery analýza MeSH
- elektrická vodivost MeSH
- elektroforéza kapilární metody MeSH
- embryo savčí * MeSH
- kultivační média MeSH
- kyselina mléčná analýza MeSH
- kyselina pyrohroznová analýza MeSH
- lidé MeSH
- limita detekce MeSH
- teplota MeSH
- Check Tag
- lidé MeSH
- Publikační typ
- časopisecké články MeSH
- práce podpořená grantem MeSH