Východiská: Zdravá výživa je dôležitým faktorom, ktorý ovplyvňuje zdravie darcu krvi ako aj kvalitu odobranej krvi. Patrí medzi faktory, ktoré môžeme zmenou stravovacích návykov ovplyvniť aj pozitívne, ale aj negatívne. Cieľom štúdie bolo posúdiť vplyv stravovania na darovanie krvi. Súbor a metódy: Štúdie sa zúčastnilo 150 bezprí- spevkových darcov krvi rôzneho pohlavia. Otázky v dotazníkoch sa zaoberali zdravotným stavom dar- cov, stravovacími návykmi pred darovaním krvi, alter- natívnym spôsobom stravovania. Dáta boli spracova- né pomocou programu Microsoft Office Excel 2007. Výsledky: Zistili sme, že 87 % respondentov pred darovaním krvi konzumovalo stravu, pričom 65 % pre- ferovalo ľahšie jedlo. Alternatívnemu spôsobu stravo- vania sa venovalo len 7 % zo všetkých respondentov, pričom 80 % nepovažovalo alternatívne stravovanie za kontraindikáciu. Záver: Dodržiavanie diétnych obmedzení pred odbe- rom krvi a pestrá, vyvážená a kvalitná strava u bezpríspevkových darcov krvi vplývalo pozitívne na ich zdravie.
Introduction: Healthy nutrition is an important fac- tor that affects the health of the blood donor as well as the quality of the collected blood. It is be- longs factors that can be influenced both positively and negatively by changing eating habits. The aim of the study was to assess the effect of diet on blood donation. File and methods: 150 blood donors of different genders participated in the study. Questions in the questionnaires dealt with the donors health state, eating habits before blood donation and an alterna- tive way of eating. Data were processed using Microsoft Office Excel 2007. Results: We found that 87 % of respondents consu- med food before blood donation while 65% preferred lighter food. Only 7 % of all respondents used an alternative way of eating, while 80% did not consider alternative eating as a contraindication. Conclusion: Adherence to dietary restrictions before blood collection and the varied, balanced and high- -quality diet in voluntary blood donors had the po- sitive effect on their health.
The HPLC method with coulochemical detection, used for the determination of selected aminothiol compounds (homocysteine, cysteine, reduced glutathione, methionine) in human plasma after quercetin supplementation by consuming cereal onion biscuits, was optimized. The effects of changes of organic modifier (acetonitrile and methanol in phosphate buffer) in the mobile phase and the pH value of the mobile phase on the retention and separation factors were studied. According to the results obtained, mobile phase containing 6 % acetonitrile (v /v) in 50 mmol L1 sodium dihydrogen phosphate solution at pH 2.6 was found to be the most suitable for the separation of selected compounds. The method was validated with respect to linearity, precision, and accuracy. Results showed that the method was sufficiently reliable, sensitive, and suitable for application to real plasma samples in clinical or epidemiological studies. Results of the pilot study pointed to the fact that the consumption of onion biscuits (with increased content of quercetine) and simultaneous drinking of green tea during two months caused decrease of homocysteine level by about 18 %. However, there were no simple correlations between quercetin and investigated parameters observed.
- MeSH
- cystein analýza krev MeSH
- funkční potraviny využití MeSH
- glutathion analýza krev MeSH
- homocystein * analýza krev MeSH
- klinická studie jako téma MeSH
- krevní plazma chemie MeSH
- lidé MeSH
- odběr vzorku krve MeSH
- reprodukovatelnost výsledků MeSH
- vysokoúčinná kapalinová chromatografie * využití MeSH
- Check Tag
- lidé MeSH
- Publikační typ
- práce podpořená grantem MeSH
HPLC determination of a background level of 8 oxodG and 2-dG in rat liver after addition of copper to perfusate was developed. The reversed phase analytical column Purospher® STAR C18e with 50 mmol L1 phosphate buffer, pH 5.5 and methanol (92:8, v/v) mobile phase was applied for the analysis. The validation of the HPLC method according to linearity, accuracy and precision was carried out. Oxidative DNA damage (expressed as concentration ratio of 8-oxodG/106 2-dG) was determined by the simultaneous measuring of 2-dG with UV detection followed by coulochemical detection of 8 oxodG. The procedure using a model of liver damage caused by intoxication with copper and ischemia / reperfusion with addition of various concentrations of CuSO4 to the perfused rat livers was tested. The aim of this study was to decide whether the toxicity of copper in liver perfusates is related to protein oxidation and oxidative DNA damage. The high contribution to the DNA damage can be related to the physical liver manipulation during harvest and reperfusion as well as to artefacts induced during the sample preparation (time-consuming sample handling during DNA isolation and extraction). The obtained results pointed out that the DNA damage occurred already during liver handling even before application of CuSO4, whereby concentration of CuSO4 higher than 0.03 mmol L1 caused a total liver damage, which led to a complete stop of the flow of the perfusate.
- MeSH
- biologické markery MeSH
- deoxyguanosin chemie MeSH
- elektrochemické techniky * metody využití MeSH
- krysa rodu rattus MeSH
- měď * toxicita MeSH
- nemoci jater * MeSH
- oxidační stres MeSH
- poškození DNA * MeSH
- spektrofotometrie metody využití MeSH
- vysokoúčinná kapalinová chromatografie * metody využití MeSH
- zvířata MeSH
- Check Tag
- krysa rodu rattus MeSH
- zvířata MeSH
- Publikační typ
- práce podpořená grantem MeSH
