Journal of immunological methods.
2008 ;
333 (1-2) :
186-191.
A phage antibody display library of single chain fragment variables (scFv) was applied to develop anti-equid herpesvirus-1 (EHV-1) glycoprotein D (gD) neutralizing antibodies. To enrich for specific scFvs, the phage antibody library was panned against epitope derived from the N-terminal part of EHV-1 gD. Unique clones were differentiated by BstNI fingerprinting and further characterized by sequencing and immunoreactivity. The neutralizing effect of each clone was assessed by plaque reduction assay. Three clones with neutralizing effect were isolated.
- MeSH
- Cell Line MeSH
- Financing, Organized MeSH
- Microscopy, Fluorescence MeSH
- Herpesvirus 1, Equid immunology MeSH
- Herpesviridae Infections immunology veterinary MeSH
- Immunoblotting MeSH
- Immunoglobulin Fragments immunology MeSH
- Kinetics MeSH
- Horses MeSH
- Molecular Sequence Data MeSH
- Horse Diseases virology MeSH
- Peptide Library MeSH
- Viral Plaque Assay MeSH
- Viral Envelope Proteins immunology MeSH
- Recombinant Proteins immunology MeSH
- Amino Acid Sequence MeSH
- Immunoglobulin Variable Region immunology MeSH
- Animals MeSH
- Check Tag
- Animals MeSH
Folia microbiologica.
2006 ;
51 (5) :
492-496.
- MeSH
- Financing, Government MeSH
- Microscopy, Fluorescence methods utilization MeSH
- Glycoproteins genetics immunology MeSH
- Herpesvirus 1, Equid immunology MeSH
- Immunoblotting methods utilization MeSH
- Antibodies, Viral genetics immunology isolation & purification MeSH
- Animals MeSH
- Check Tag
- Animals MeSH
