Our previous studies have shown that the combined administration of drugs elevating extracellular adenosine, i.e. dipyridamole (DP) and adenosine monophosphate (AMP), enhances murine hematopoiesis and potentiates the action of granulocyte colony-stimulating factor (G-CSF). In this study, colony-stimulating activity (CSA) of blood serum of mice treated with DP+AMP, G-CSF or all these drugs in combination, i.e. the ability of the sera to stimulate the growth of GM-CFC colonies, was assayed in vitro. Furthermore, the concentration of GM-CSF and IL-6 in the sera was determined. Administration of DP+AMP was found to enhance significantly serum CSA at all time intervals of serum sampling including 24 h after the last injection of the tested drugs. Additive effects of DP+AMP and G-CSF on serum CSA were noted at early intervals after administration of the drugs. Furthermore, IL-6 levels were significantly elevated in the sera of mice which were administered DP+AMP either alone or in combination with G-CSF. Our results show that the effects of DP+AMP are indirect, mediated through the induction of some cytokine(s) and/or growth factor(s) and that extracellular adenosine can act in cooperation with G-CSF. These findings contribute to the further elucidation of the role of adenosine in hematopoiesis.

• MeSH
• adenosin analogy a deriváty   imunologie   MeSH
• adenosinmonofosfát aplikace a dávkování   krev   MeSH
• dipyridamol aplikace a dávkování   krev   MeSH
• ELISA normy   využití   MeSH
• extracelulární prostor genetika   imunologie   účinky léků   MeSH
• faktor stimulující kolonie granulocytů izolace a purifikace   účinky léků   MeSH
• financování organizované využití   MeSH
• myši inbrední CBA krev   MeSH
• myši inbrední ICR krev   MeSH

• MeSH
• adenosin farmakologie   metabolismus   MeSH
• antimetabolity toxicita   MeSH
• dipyridamol farmakologie   terapeutické užití   MeSH
• faktor stimulující kolonie granulocytů farmakologie   terapeutické užití   MeSH
• fluoruracil toxicita   MeSH
• hemoglobiny metabolismus   účinky léků   MeSH
• inhibitory fosfodiesteras farmakologie   terapeutické užití   MeSH
• myši MeSH
• zvířata MeSH
• Check Tag
• myši MeSH
• zvířata MeSH

• MeSH
• antagonisté prostaglandinu MeSH
• flurbiprofen analogy a deriváty   farmakologie   MeSH
• hematopoéza účinky léků   účinky záření   MeSH
• myši MeSH
• prostaglandiny MeSH
• radiační poranění farmakoterapie   MeSH
• radioprotektivní látky MeSH
• zvířata MeSH
• Check Tag
• myši MeSH
• zvířata MeSH
• Publikační typ
• srovnávací studie MeSH