Assembly of immature retroviral particles is a complex process involving interactions of several specific domains of the Gag polyprotein localized mainly within capsid protein (CA), spacer peptide (SP), and nucleocapsid protein (NC). In the present work we focus on the contribution of NC to the oligomerization of CA leading to assembly of Mason-Pfizer monkey virus (M-PMV) and HIV-1. Analyzing in vitro assembly of substitution and deletion mutants of DeltaProCANC, we identified a "spacer-like" sequence (NC(15)) at the M-PMV NC N terminus. This NC(15) domain is indispensable for the assembly and cannot be replaced with oligomerization domains of GCN4 or CREB proteins. Although the M-PMV NC(15) occupies a position analogous to that of the HIV-1 spacer peptide, it could not be replaced by the latter one. To induce the assembly, both M-PMV NC(15) and HIV-1 SP1 must be followed by a short peptide that is rich in basic residues. This region either can be specific, i.e., derived from the downstream NC sequence, or can be a nonspecific positively charged peptide. However, it cannot be replaced by heterologous interaction domains either from GCN4 or from CREB. In summary, we report here a novel M-PMV spacer-like domain that is functionally similar to other retroviral spacer peptides and contributes to the assembly of immature-virus-like particles.
- MeSH
- buněčné linie MeSH
- DNA primery genetika MeSH
- DNA virů genetika MeSH
- Escherichia coli genetika ultrastruktura virologie MeSH
- HIV-1 fyziologie genetika MeSH
- lidé MeSH
- Masonův-Pfizerův opičí virus fyziologie genetika ultrastruktura MeSH
- molekulární sekvence - údaje MeSH
- multimerizace proteinu MeSH
- mutageneze MeSH
- nukleokapsida - proteiny fyziologie genetika chemie MeSH
- rekombinantní proteiny genetika chemie metabolismus MeSH
- sekvence aminokyselin MeSH
- sekvence nukleotidů MeSH
- sekvenční homologie aminokyselin MeSH
- sestavení viru fyziologie genetika MeSH
- terciární struktura proteinů MeSH
- transmisní elektronová mikroskopie MeSH
- zvířata MeSH
- Check Tag
- lidé MeSH
- zvířata MeSH
- Publikační typ
- práce podpořená grantem MeSH
- Research Support, N.I.H., Extramural MeSH
- MeSH
- aktiny biosyntéza MeSH
- buněčná membrána imunologie ultrastruktura MeSH
- finanční podpora výzkumu jako téma MeSH
- lidé MeSH
- mastocyty imunologie ultrastruktura MeSH
- receptory IgE imunologie MeSH
- signální transdukce MeSH
- test degranulace bazofilů MeSH
- transdukce genetická MeSH
- zvířata MeSH
- Check Tag
- lidé MeSH
- zvířata MeSH
- MeSH
- buněčná diferenciace fyziologie MeSH
- buňky HT-29 MeSH
- butyráty metabolismus MeSH
- glukosa metabolismus MeSH
- lidé MeSH
- Check Tag
- lidé MeSH
