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- MeSH
- hokej dějiny MeSH
- osobní vzpomínky jako téma MeSH
- Publikační typ
- práce podpořená grantem MeSH
- Geografické názvy
- Československo MeSH
The intracellular pathogens have the unique capacity to sense the host cell environment and to respond to it by alteration in gene expression and protein synthesis. Proteomic analysis of bacteria exposed directly to the host cell milieu might thus greatly contribute to the elucidation of processes leading to bacterial adaptation and proliferation inside the host cell. Here we have performed a global proteome analysis of a virulent Francisella tularensis subsp. holarctica strain during its intracellular cycle within the macrophage-like murine cell line J774.2 using the metabolic pulse-labeling of bacterial proteins with (35)S-methionine and (35)S-cysteine in various periods of infection. The two-dimensional gel analysis revealed macrophage-induced bacterial proteome changes in which 64 identified proteins were differentially expressed in comparison to controls grown in tissue culture medium. Nevertheless, activation of macrophages with interferon gamma before in vitro infection decreased the number of detected alterations in protein levels. Thus, these proteomic data indicate the F. tularensis ability to adapt to the intracellular hostile environment that is, however, diminished by prior interferon gamma treatment of host cells.
- MeSH
- 2D gelová elektroforéza MeSH
- bakteriální proteiny chemie genetika metabolismus MeSH
- Francisella tularensis chemie genetika fyziologie MeSH
- interakce hostitele a patogenu * MeSH
- lidé MeSH
- makrofágy mikrobiologie MeSH
- molekulární sekvence - údaje MeSH
- myši MeSH
- proteomika MeSH
- tularemie mikrobiologie MeSH
- zvířata MeSH
- Check Tag
- lidé MeSH
- myši MeSH
- zvířata MeSH
- Publikační typ
- časopisecké články MeSH
- práce podpořená grantem MeSH
- srovnávací studie MeSH
- MeSH
- 2D gelová elektroforéza metody přístrojové vybavení využití MeSH
- automatizované zpracování dat metody normy MeSH
- Bacteria MeSH
- bakteriologické techniky metody využití MeSH
- Borrelia burgdorferi komplex genetika imunologie MeSH
- Borrelia genetika imunologie MeSH
- klíště MeSH
- lymeská nemoc diagnóza etiologie mikrobiologie MeSH
- proteiny izolace a purifikace MeSH
- proteomika metody MeSH
- spektrometrie hmotnostní - ionizace laserem za účasti matrice metody přístrojové vybavení využití MeSH
Lymeská borelióza je vážným onemocněním, jehož původcem je v Evropě nejčastěji Borrelia garinii. Pro pochopení patogeneze tohoto onemocnění je důležité získat informace o proteinovém složení původce a významným zdrojem těchto informací je proteomová analýza. Pro identifikaci pomocí dvojrozměrové elektroforézy a hmotnostní spektrometrie bylo vybráno 63 proteinových spot Borrelia garinii (kmen CB61). Z nich bylo úspěšně identifikováno 40, většina se však vyskytovala v různých izoformách. Identifikováno tak bylo celkem 16 různých proteinů. Převažují proteiny s enzymatickou funkcí, ale podařilo se také identifikovat některé proteiny zahrnuté v enzootickém cyklu. Jsou to zejména proteiny skupiny Osp, membránový protein p66 a flagelární protein.
Lyme disease is a serious illness caused in Europe most frequently by Borrelia garinii. It is important to find information about protein content for understanding pathogenesis of Lyme disease. Proteome analysis is an important source of this information. We used 2-dimensional electrophoresis and mass spectrometry for identification of 63 chosen Borrelia garinii (strain CB61) protein spots. Overall 40 protein spots were identified however most of them occurred in several isoforms. Totally 16 different proteins were identified. Among them we identified several enzymes. Proteins that participate in enzootic cycle of pathogen are more interesting. They are mainly proteins of Osp group, membrane protein p66 and flagellar protein.
- MeSH
- 2D gelová elektroforéza metody využití MeSH
- biologické markery MeSH
- Borrelia burgdorferi komplex genetika izolace a purifikace MeSH
- hmotnostní spektrometrie metody využití MeSH
- klíště mikrobiologie parazitologie patogenita MeSH
- lidé MeSH
- lymeská nemoc genetika imunologie mikrobiologie MeSH
- proteomika metody trendy MeSH
- vakcína proti lymeské nemoci genetika imunologie MeSH
- virulence genetika imunologie MeSH
- Check Tag
- lidé MeSH
Francisella tularensis is a highly virulent, facultative intracellular pathogen that causes tularemia in humans and animals. Although it is one of the most infectious bacterial pathogens, little is known about its virulence mechanisms. In this study, the response of F. tularensis live vaccine strain to iron depletion, which simulates the environment within the host, was investigated. In order to detect alterations in protein synthesis, metabolic labeling, followed by 2D-PAGE analysis was used. Globally, 141 protein spots were detected whose levels were significantly altered in the iron-restricted medium. About 65% of the spots were successfully identified using mass spectrometric approaches. Importantly, among the proteins produced at an increased level during iron-limited growth, three proteins were found encoded by the igl operon, located in the F. tularensis pathogenicity island I (FPI). Of these, the IglC and IglA proteins were previously reported to be necessary for full virulence of F. tularensis. These results, obtained at the proteome level, support and confirm recently published data showing that the igl operon genes are transcribed in response to iron limitation
- MeSH
- 2D gelová elektroforéza MeSH
- bakteriální proteiny genetika metabolismus MeSH
- faktory virulence genetika metabolismus MeSH
- financování organizované MeSH
- Francisella tularensis metabolismus patogenita růst a vývoj MeSH
- genomové ostrovy MeSH
- hmotnostní spektrometrie MeSH
- operon MeSH
- proteomika MeSH
- regulace genové exprese u bakterií MeSH
- stanovení celkové genové exprese MeSH
- železo metabolismus MeSH
