Thirty-five Staphylococcus aureus strains from auricular infections were isolated. The identification of strains was confirmed by Api ID 32 Staph strips, the antibiotic susceptibility test was performed using ATB Staph kit. PCR assay was used to detect the oxacillin resistance gene (mecA) and the erythromycin genes (ermA, ermB, ermC, msrA and mef). The susceptibility profile of all strains revealed a low resistance level to oxacillin and erythromycin. The PCR results show that 60 % of the strains are mecA positive. The frequency of erythromycin genes was: ermA (+) 22.8 %, ermB (+) 45.7, ermC (+) 17.1, msrA (+) 28.6. The mef gene was not detected in any strain. No correlations between genotypic and phenotypic methods for the determination of oxacillin and erythromycin resistance was found. However, multiplex PCR technique was shown to be a fast, practical and economic technique for the detection of methicillin-and erythromycin-resistant staphylococci.

• MeSH
• antibakteriální látky farmakologie   MeSH
• bakteriální léková rezistence MeSH
• bakteriální proteiny genetika   metabolismus   MeSH
• erythromycin farmakologie   MeSH
• lidé MeSH
• membránové proteiny genetika   metabolismus   MeSH
• methyltransferasy genetika   metabolismus   MeSH
• oxacilin farmakologie   MeSH
• polymerázová řetězová reakce metody   MeSH
• stafylokokové infekce mikrobiologie   MeSH
• Staphylococcus aureus genetika   izolace a purifikace   účinky léků   MeSH
• ušní boltec mikrobiologie   MeSH
• Check Tag
• lidé MeSH