Several irritants were used in the in vitro irritation medical device round robin. The objective of this study was to verify their irritation potential using the human patch test (HPT), an in vitro assay, and in vivo data. The irritants were lactic acid (LA), heptanoic acid (HA), sodium dodecyl sulfate (SDS), Genapol® X-80 (GP), and Y-4 polymer. Dilute saline and sesame seed oil (SSO) solutions of each were evaluated using a 4 and 18 h HPT and the EpiDerm™ SIT-MD RhE assay; results were then compared to existing rabbit skin irritation test data. Results from the 4 h HPT were negative in most cases except for GP and SDS, while the 18 h HPT also identified some LA, HA, and GP samples as irritants. EpiDerm™ SIT-MD correctly identified all irritants except GP in SSO due to limited solubility. Data from cutaneous rabbit irritation tests were negative, while all intracutaneous results were strongly or weakly positive except for the most dilute GP solutions. These findings indicate that EpiDerm™ SIT-MD results correlate with those from the rabbit intracutaneous test and confirm that RhE assays are suitable replacements for animals in evaluating the tissue irritation potential of medical devices.

• MeSH
• alternativy testů na zvířatech MeSH
• benchmarking MeSH
• dodecylsíran sodný toxicita   MeSH
• dráždivé látky toxicita   MeSH
• králíci MeSH
• kůže účinky léků   MeSH
• kyselina mléčná toxicita   MeSH
• kyseliny heptylové toxicita   MeSH
• lidé MeSH
• náplasťové testy metody   MeSH
• polyethylenglykoly toxicita   MeSH
• polyvinylchlorid toxicita   MeSH
• reprodukovatelnost výsledků MeSH
• testy kožní dráždivosti metody   MeSH
• zdravotnické prostředky * MeSH
• zvířata MeSH
• Check Tag
• králíci MeSH
• lidé MeSH
• zvířata MeSH
• Publikační typ
• časopisecké články MeSH