The effect of recombinant divercin RV41 (DvnRV41) and its structural variants on the K-channel formation was determined. The growth of Listeria monocytogenes EGDe (sensitive phenotype) and its isogenic strain (resistant phenotype) was assessed in the presence of DvnRV41 combined or not with pinacidil, NS1619, cromakalim (as K-channel activators), iberiotoxin and glipizide (as K-channel blockers). The combined action of DvnRV41 and K activators permitted formation of ATP-dependent pores. The combination of DvnRV41 and ATP-dependent pore activator cromakalim inhibited the growth of sensitive strain. The antilisterial activity of structural variants was less important than that of DvnRV41 but their mode of action remained overall similar.
- MeSH
- adenosintrifosfát metabolismus MeSH
- antibakteriální látky metabolismus MeSH
- bakteriociny genetika metabolismus MeSH
- blokátory draslíkových kanálů metabolismus MeSH
- draslíkové kanály agonisté metabolismus MeSH
- Listeria monocytogenes účinky léků genetika růst a vývoj metabolismus MeSH
- mikrobiální testy citlivosti MeSH
- rekombinantní proteiny genetika metabolismus MeSH
- Publikační typ
- časopisecké články MeSH
- práce podpořená grantem MeSH
It was shown recently (Calvez et al. 2007) that glpQ and pde genes are involved in intermediate resistance of Enterococcus faecalis JH2-2 to DvnV41, a class IIa bacteriocin produced by Carnobacterium divergens V41. The listerial orthologs of lpQ and pde genes might be lmo0052 and lmo1292 genes, respectively. Here, the role of lmo0052 and lmo1292 genes in resistance of Listeria monocytogenes EGDe to DvnV41 and MesY105 was investigated. L. monocytogenes EGDe was inactivated in lmo0052 and/or lmo1292 by homologous recombination. Listerial mutant strain EGDSC02 (inactivated in the putative glpQ gene), was slightly resistant to DvnV41. The listerial mutant strain EGDSC01 (inactivated in the putative pde gene) remained, as the wild-type strain, sensitive to DvnV41, but was affected in growth parameters.
- MeSH
- antibakteriální látky farmakologie MeSH
- bakteriální léková rezistence MeSH
- bakteriální proteiny genetika účinky léků MeSH
- bakteriociny biosyntéza farmakologie MeSH
- fosfodiesterasy genetika účinky léků MeSH
- Listeria monocytogenes genetika růst a vývoj účinky léků MeSH
- mikrobiální testy citlivosti MeSH
- mutace MeSH
- MeSH
- bakteriociny izolace a purifikace MeSH
- chromatografie afinitní metody využití MeSH
- DNA genetika klasifikace MeSH
- ELISA metody využití MeSH
- Enterococcus faecalis enzymologie genetika MeSH
- Escherichia coli genetika imunologie MeSH
- financování vládou MeSH
- lidé MeSH
- polymerázová řetězová reakce metody využití MeSH
- Check Tag
- lidé MeSH
