Hyaluronan-based tissue substitutes are promising materials in cartilage reconstruction surgery. Herein, the chondrogenesis of human mesenchymal stem cells (MSC) in a hydrogel based on a tyramine derivative of hyaluronan crosslinked by hydrogen peroxidase (HA-TA) was evaluated. Human MSC seeded in the scaffold were incubated in standard chondrogenic medium and medium enriched with bone morphogenetic protein-6 (BMP6). Cell viability, the gene expression of selected markers (collagen type II, aggrecan, SOX9, collagen type X, and osteopontin), and the histological characteristics were examined during three weeks of in vitro cultivation. The tissue reaction of both unseeded and MSC seeded HA-TA scaffolds were tested in vivo after subcutaneous application in rats for 12 weeks. The data showed that cells resisted the process of crosslinking and remained viable for the whole time while exhibiting changes in cell organization. Human MSC cultivated in HA-TA hydrogel expressed genes of both chondrogenic and osteogenic differentiation and the addition of BMP6 revealed a tendency to potentiate both processes. Histological analysis of HA-TA in vivo implants did not reveal a chronic inflammatory reaction. In both cases, in vivo HA-TA implants were continuously degraded and MSC-seeded hydrogels tended to form clusters similar to in vitro samples. In conclusion, MSC chondrogenic differentiation may proceed in a HA-TA scaffold that is biocompatible. © 2013 Wiley Periodicals, Inc. J Biomed Mater Res Part A: 102A: 3523-3530, 2014.
- MeSH
- buněčná diferenciace * účinky léků genetika MeSH
- chondrogeneze * účinky léků genetika MeSH
- experimentální implantáty MeSH
- imobilizované buňky cytologie účinky léků metabolismus MeSH
- krysa rodu rattus MeSH
- kyselina hyaluronová farmakologie MeSH
- lidé MeSH
- mezenchymální kmenové buňky cytologie účinky léků metabolismus MeSH
- PEG-DMA hydrogel farmakologie MeSH
- peroxidasa metabolismus MeSH
- reagencia zkříženě vázaná farmakologie MeSH
- regulace genové exprese účinky léků MeSH
- subkutánní tkáň účinky léků MeSH
- tyramin farmakologie MeSH
- viabilita buněk účinky léků MeSH
- zobrazování trojrozměrné MeSH
- zvířata MeSH
- Check Tag
- krysa rodu rattus MeSH
- lidé MeSH
- zvířata MeSH
- Publikační typ
- časopisecké články MeSH
- práce podpořená grantem MeSH
- MeSH
- časové faktory MeSH
- diabetes mellitus 1. typu * genetika terapie MeSH
- diabetická retinopatie * epidemiologie genetika MeSH
- genetická variace MeSH
- glykovaný hemoglobin * genetika MeSH
- kohortové studie MeSH
- laserová terapie MeSH
- lidé MeSH
- rizikové faktory MeSH
- Check Tag
- lidé MeSH
- Publikační typ
- komentáře MeSH
- souhrny MeSH
