BACKGROUND/AIM: Serum thymidine kinase 1 (STK1) is a proliferation biomarker that has been used as a diagnostic marker of several malignant diseases. However, there are limited data for prostate cancer (PCa). PATIENTS AND METHODS: In this study, we retrospectively analysed serum samples from 169 patients with biopsy confirmed PCa, who had been indicated for radical prostatectomy (RP) between 2013-2016. The results were compared with those in serum samples from 39 healthy men. We used commercially available enzymatic immunoassay to determine the levels of STK1. The patients were divided into groups according to the Gleason score (GS) and risk factors for adjuvant radiotherapy (aRT), which were defined as GS 8-10, pT3, and a positive surgical margin. RESULTS: The median serum level of STK1 in PCa patients was 0.289 pmol/l. In the control group, the median value was 0.0116 pmol/l (p<0.001). By comparing the patients with GS≤6 vs. 7 vs. ≥8 (p=0.01), we found statistically significant differences. In the correlation of STK1 values with risk factors, we found statistically significant differences both in comparison of 0 vs. 1 vs. 2 vs. 3 risk factors (p=0.021), as well as ≤1 vs. 2≥ risk factors (p=0.009). CONCLUSION: The levels of STK1 are significantly higher in patients with PCa than those in healthy controls. Furthermore, STK1 values correlate with GS and predefined risk factors for aRT. Therefore, STK1 can be considered as a potential tumour marker of PCa diagnosis and risk stratification.
- MeSH
- lidé MeSH
- nádorové biomarkery * MeSH
- nádory prostaty * patologie MeSH
- prostata patologie MeSH
- prostatektomie MeSH
- retrospektivní studie MeSH
- stupeň nádoru MeSH
- thymidinkináza MeSH
- Check Tag
- lidé MeSH
- mužské pohlaví MeSH
- Publikační typ
- časopisecké články MeSH
PURPOSE OF THE STUDY Hyaluronic acid is a major component of synovial fluid and an indicator of joint viscosity and viscoelasticity. Decreased concentrations of hyaluronic acid in the synovial fluid indicate the severity of osteoarthritis. The aim of the study was to determine the concentrations of hyaluronic acid in the synovial fluid and to evaluate the correlation with the severity of osteoarthritis determined based on the radiological criteria (evaluation according to X-ray classification) and the physicochemical criteria (measurement of synovial fluid osmolality). MATERIAL AND METHODS A total of 67 patients with the mean age of 67 years (35 men, mean age = 65 years, and 32 women, mean age = 68 years) with clinically diagnosed osteoarthritis were included in the study. The patients were divided into four groups according to the severity of arthrosis (from Group 1 - the lowest degree of disability to Group 4 - the most severe degree of arthrosis). Samples were taken during knee arthroscopies or total knee replacements. Hyaluronic acid concentrations in synovial fluid were determined using a commercially available kit based on the sandwich ELISA principle (Teco Hyaluronic acid, Switzerland). Osmolality was measured by the cryoscopic method. RESULTS The average concentrations of hyaluronic acid in the synovial fluid in the individual groups were as follows: Group 1: HA = 2302 mg/L, Group 2: HA = 2234 mg/L, Group 3: HA = 2300 mg/L, Group 4: HA = 2446 mg/L. HA concentrations in synovial fluid did not differ from the published reference values in synovial fluid (1500 - 3200 mg/L. HA concentrations do not depend on age, sex, and severity of the disease. The patients who underwent arthroscopy have significantly elevated synovial HA concentration compared to the patients who underwent total knee replacements (2581 mg/l/L vs. 1763 mg/l/L, p = 0.01, Mann-Whitney test). HA positively correlates with the osmotic pressure determined by the examination of osmolality in synovial fluid (r = 0.29, p = 0.015). CONCLUSIONS Hyaluronic acid concentrations in patients with knee osteoarthritis who underwent arthroscopy are significantly increased compared to the group of patients with total knee replacement. Hyaluronic acid concentrations in the synovial fluid correlate with the increased osmotic pressure, and may therefore be an additional indicator of the severity of the disease. Key words: hyaluronic acid, knee arthrosis, total knee replacement, osmolality.
- MeSH
- artróza kolenních kloubů * diagnóza chirurgie MeSH
- kolenní kloub diagnostické zobrazování chirurgie MeSH
- kyselina hyaluronová MeSH
- lidé MeSH
- senioři MeSH
- synoviální tekutina MeSH
- totální endoprotéza kolene * MeSH
- Check Tag
- lidé MeSH
- mužské pohlaví MeSH
- senioři MeSH
- ženské pohlaví MeSH
- Publikační typ
- časopisecké články MeSH
The aim of the study was to investigate the hyaluronic acid concentration in middle ear fluid of patients with cleft palate as an indicator of the severity of the disease. Hyaluronic acid was examined in the middle ear fluid of 65 children (48 boys and 17 girls) subjected to cleft lip surgery in neonatal period up to 10 days of age. Patients were divided into 3 groups according to the course of the disease. First group consists of 15 patients with favorable course, second group consist of 25 patients with moderate course, third group included 25 patients with an adverse course. Hyaluronic acid levels were determined by commercially available immunoassay. The concentrations of hyaluronic acid in the middle ear fluid were as follows (mean+SEM): favorable course: 14253+2393 μg/l, moderate course: 7503+1345 μg/l, adverse course: 5905+2393 μg/l. Patients with adverse course and moderate course had significantly decreased hyaluronic acid levels in middle ear fluid compared to the patients with favorable course (P=0.02 and P=0.0018). Hyaluronic acid concentration is related to the course of the disease and the lowest values are most frequent in patients with an adverse course.
- MeSH
- imunoanalýza metody MeSH
- kyselina hyaluronová analýza MeSH
- lidé MeSH
- novorozenec MeSH
- otitis media s výpotkem komplikace diagnóza MeSH
- posouzení stavu pacienta MeSH
- rozštěp patra komplikace MeSH
- střední ucho chemie MeSH
- tělesné tekutiny chemie MeSH
- Check Tag
- lidé MeSH
- mužské pohlaví MeSH
- novorozenec MeSH
- ženské pohlaví MeSH
- Publikační typ
- časopisecké články MeSH
Background: It has been shown that decreased expression and activity of extracellular matrix protein mindin correlate with various types of cancers including breast, colon and lung cancers. The aim of the presented study was to investigate the serum mindin levels in prostate cancer. Methods: Mindin concentrations in serum were measured in 56 patients with prostate cancer (mean age 68 years) and in control group of 29 healthy men (mean age 64 years) using commercially available enzymatic immunoassay (Cusabio, WuHan, China). The patients were divided with respect to the severity of the disease into two groups according to the EAU guidelines (stage 1, 2 - less severe tumours, stage 3, 4 - severe tumours). Results: Serum mindin concentrations were significantly elevated in the group of healthy individuals unlike in the patients with prostate cancer (2.12 ng/mL vs 0.78 ng/mL, with P=0.0007, AUC=0.705). Patients with less severe tumours (stage 1, 2) and severe tumours (stage 3, 4) had significantly decreased levels of S-mindin as well (P=0.0037), although the difference in serum mindin concentrations between the patients with less severe and severe tumours was not significant. Conclusions: Concentrations of mindin were decreased in patients with prostate cancer and reduced in patients with less severe prostate cancer as well. Mindin appears to be a promising diagnostic marker useful in the diagnosis of prostate cancer.
- Publikační typ
- časopisecké články MeSH
Úvod: Téměř všichni pacienti s rozštěpem patra trpí insuficiencí Eustachovy trubice. Následkem bývá rozvoj sekretorické otitidy. Její první projevy lze pozorovat již u novorozenců. Cílem práce je zachytit první příznaky onemocnění v rámci první fáze chirurgického řešení – primární sutury rtu, kterou provádíme v novorozeneckém období. Dalším cílem je zhodnocení výpovědní hodnoty objektivních audiologických metod ve vztahu k diagnostice středoušního sekretu. Materiál a metodika: V desetiletém období byl hodno-cen soubor 314 pacientů s rozštěpovou vadou, u kterých byla provedena primární sutura rtu v novorozeneckém období. Před výkonem byly vyšetřeny OAE, standardní 226 Hz a vysokofrekvenční 1 000 Hz tympanometrie. Ne u všech pacientů se podařilo provést všechna vyšetření současně. V případě podezření na přítomnost středoušního sekretu byla provedena otomikroskopie s diagnostickou paracentézou a případným odsátím sekretu. Výsledky: Během deseti let byla provedena primární sutura rtu u 314 novorozenců, z toho 241 do sedmého dne věku. Nejvíce pacientů (59) bylo operováno třetí poporodní den. 178 pacientů mělo současně rozštěp rtu a patra, 136 pouze rozštěp rtu. Celkem bylo vyšetřeno 394 uší 1 000 Hz sondou a 408 standardní 226 Hz sondou. Středoušní sekret byl zachycen u 74 % pacientů s rozštěpem patra (jednostranně nebo oboustranně). Sekret se pravidelně objevoval i na nerozštěpové straně, kde byl normálně morfologicky konfigurovaný tubární torus. V 96 % se shodoval výsledek 1 000 Hz tympanometrického vyšetření (B křivka) s nálezem sekretu. Závěr: Středoušní sekret byl zachycen již u novorozenců, ale pouze u těch, kteří měli rozštěp patra. U všech pacientů s izolovaným rozštěpem rtu bylo středouší vzdušné. Přítomnost sekretu nezávisí na straně rozštěpu. Vysokofrekvenční tympanometrie je vysoce průkazná metoda ke stanovení přítomnosti středoušního sekretu u novorozenců.
Introduction: Almost all patients with cleft palate suffer from Eustachian tube insufficiency.The result is usually the development of otitis media with effusion. Its first manifestation can be observed in newborns. The aim of this work is to detect the first symptoms of the disease during the first phase of the surgical solution - the primary suture of the lip, which we perform in neo-neonatal age. Another goal is to evaluate the value of objective audiological methods in relation to the diagnosis of middle ear fluid. Material and methods: In a ten-year period, a group of 314 patients with cleft defects were evaluated, who had primary lip suture in the neonatal period. Prior to the procedure, OAE, standard 226 Hz and high frequency 1000 Hz tympanometry were examined. However, it was not possible to complete all examinations in all patients. In case of suspicion of presence of middle ear secretion, otomicroscopy with diagnostic paracentesis was performer. Results: Over ten years, lip surgery was performed in 314 newborns, 241 to 7 days of age. Most patients (57) were operated on the third postpartum day. 178 patients had cleft lip and palate, 136 patients had cleft lip. A total of 394 ears with a 1000 Hz probe and a 408 standard 226 Hz probe were examined. Middle ear fluid was detected in 74% of patients with cleft palate (unilaterally or bilaterally). The middel ear fluid appeared regularly on the non-cleft side, where the tubular torus was normally morphologically configured. In 96% the result of 1000 Hz tympanometric examination (B curve) coincided with the finding of middle ear fluid. Conclusion: Middle ear secretion was detected in newborns, but only in those who had cleft palate. In all patients with isolated cleft lip, the middle ear was airy. The presence of secretion does not depend on the cleft side. High-frequency tympanometry is a highly conclusive method for determining the presence of middle ear fluid in newborns.
