- MeSH
- buněčná imunita účinky léků MeSH
- COVID-19 * mortalita prevence a kontrola MeSH
- dezinformace MeSH
- karanténa MeSH
- lidé MeSH
- mRNA vakcíny MeSH
- vakcíny proti COVID-19 MeSH
- všeobecná preventivní opatření metody MeSH
- Check Tag
- lidé MeSH
- Geografické názvy
- Česká republika MeSH
- Evropa MeSH
7., aktualizované vydání 374 stran : ilustrace (převážně barevné) ; 20 cm
Vysokoškolská učebnice, která se zaměřuje na imunologii.
- Konspekt
- Patologie. Klinická medicína
- Učební osnovy. Vyučovací předměty. Učebnice
- NLK Obory
- alergologie a imunologie
- NLK Publikační typ
- učebnice vysokých škol
Styrene-maleic acid (SMA) and similar amphiphilic copolymers are known to cut biological membranes into lipid nanoparticles/nanodiscs containing membrane proteins apparently in their relatively native membrane lipid environment. Our previous work demonstrated that membrane raft microdomains resist such disintegration by SMA. The use of SMA in studying membrane proteins is limited by its heterogeneity and the inability to prepare defined derivatives. In the present paper, we demonstrate that some amphiphilic peptides structurally mimicking SMA also similarly disintegrate cell membranes. In contrast to the previously used copolymers, the simple peptides are structurally homogeneous. We found that their membrane-disintegrating activity increases with their length (reaching optimum at 24 amino acids) and requires a basic primary structure, that is, (XXD)n, where X represents a hydrophobic amino acid (optimally phenylalanine), D aspartic acid, and n is the number of repeats of these triplets. These peptides may provide opportunities for various well-defined potentially useful modifications in the study of membrane protein biochemistry. Our present results confirm a specific character of membrane raft microdomains.
- MeSH
- buněčná membrána metabolismus chemie MeSH
- buněčné linie MeSH
- lidé MeSH
- maleáty chemie MeSH
- membránové mikrodomény metabolismus chemie MeSH
- membránové proteiny * chemie metabolismus MeSH
- peptidy * chemie MeSH
- polystyreny chemie MeSH
- zvířata MeSH
- Check Tag
- lidé MeSH
- zvířata MeSH
- Publikační typ
- časopisecké články MeSH
- práce podpořená grantem MeSH
An advantageous alternative to the use of detergents in biochemical studies on membrane proteins are the recently developed styrene-maleic acid (SMA) amphipathic copolymers. In our recent study [1] we demonstrated that using this approach, most T cell membrane proteins were fully solubilized (presumably in small nanodiscs), while two types of raft proteins, GPI-anchored proteins and Src family kinases, were mostly present in much larger (>250 nm) membrane fragments markedly enriched in typical raft lipids, cholesterol and lipids containing saturated fatty acid residues. In the present study we demonstrate that disintegration of membranes of several other cell types by means of SMA copolymer follows a similar pattern and we provide a detailed proteomic and lipidomic characterization of these SMA-resistant membrane fragments (SRMs).
Chimeric antigen receptor (CAR)-expressing T-cells are without a doubt a breakthrough therapy for hematological malignancies. Despite their success, clinical experience has revealed several challenges, which include relapse after targeting single antigens such as CD19 in the case of B-cell acute lymphoblastic leukemia (B-ALL), and the occurrence of side effects that could be severe in some cases. Therefore, it became clear that improved safety approaches, and targeting multiple antigens, should be considered to further improve CAR T-cell therapy for B-ALL. In this paper, we address both issues by investigating the use of CD10 as a therapeutic target for B-ALL with our switchable UniCAR system. The UniCAR platform is a modular platform that depends on the presence of two elements to function. These include UniCAR T-cells and the target modules (TMs), which cross-link the T-cells to their respective targets on tumor cells. The TMs function as keys that control the switchability of UniCAR T-cells. Here, we demonstrate that UniCAR T-cells, armed with anti-CD10 TM, can efficiently kill B-ALL cell lines, as well as patient-derived B-ALL blasts, thereby highlighting the exciting possibility for using CD10 as an emerging therapeutic target for B-cell malignancies.
- MeSH
- antigeny CD19 metabolismus MeSH
- chronická lymfatická leukemie * metabolismus terapie MeSH
- imunoterapie adoptivní MeSH
- leukemie B-buněčná * metabolismus terapie MeSH
- lidé MeSH
- neprilysin * terapeutické užití MeSH
- receptory antigenů T-buněk metabolismus MeSH
- T-lymfocyty MeSH
- Check Tag
- lidé MeSH
- Publikační typ
- časopisecké články MeSH
- MeSH
- COVID-19 * imunologie přenos prevence a kontrola MeSH
- lidé MeSH
- vakcíny proti COVID-19 MeSH
- Check Tag
- lidé MeSH
Monoklonální protilátky se staly jedním z nejdůležitějších protinádorových terapeutických prostředků. Jedním z mechanismů jejich účinku je buněčná cytotoxicita zprostředkovaná protilátkami (ADCC). Nádorové buňky opsonizované protilátkami mohou být cytotoxicky likvidovány imunitními buňkami vybavenými Fc receptory (NK a NKT lymfocyty, většina myeloidních buněk). Tento mechanismus se uplatňuje paralelně s ostatními známými mechanismy (fagocytóza opsonizovaných buněk, lýza po aktivaci klasické dráhy komplementu, blokování funkce receptorů růstových faktorů, blokování tlumivých „kontrolních bodů ).
- MeSH
- buněčná cytotoxicita závislá na protilátkách * fyziologie účinky léků MeSH
- buňky NK fyziologie MeSH
- cílená molekulární terapie * metody MeSH
- humorální imunita fyziologie účinky léků MeSH
- imunoglobuliny fyziologie imunologie MeSH
- inhibitory kontrolních bodů farmakologie MeSH
- lidé MeSH
- monoklonální protilátky farmakologie imunologie MeSH
- nádory farmakoterapie imunologie MeSH
- receptory Fc fyziologie MeSH
- Check Tag
- lidé MeSH
