A validated, highly sensitive and selective high-pressure liquid chromatography-tandem mass spectrometry (LC-MS/MS) method was developed for the quantitative determination of quetiapine (QUE) in human Na2EDTA plasma with mass spectrometry (MS) detection. Clozapine (CLO) was employed as an internal standard. Samples were extracted using solid phase extraction (SPE). Oasis HLB cartridges and the concentration of quetiapine was determined by isocratic HPLC-MS/MS. The SRM mode was used for MS/MS detection. The method was validated over a concentration range of 1.0-382.2 ng/mL. Inter- and intra-day precision and accuracy of the proposed method were characterized by relative standard deviation (R.S.D.) and the percentage of deviation, respectively; both were lower than 8%. The developed method was employed in the pharmacokinetic study of quetiapine.
- MeSH
- antipsychotika farmakokinetika krev MeSH
- chromatografie kapalinová MeSH
- dibenzothiazepiny farmakokinetika krev MeSH
- hmotnostní spektrometrie MeSH
- kalibrace MeSH
- lidé MeSH
- plocha pod křivkou MeSH
- referenční standardy MeSH
- reprodukovatelnost výsledků MeSH
- řízení kvality MeSH
- stabilita léku MeSH
- terapeutická ekvivalence MeSH
- vysokoúčinná kapalinová chromatografie MeSH
- Check Tag
- lidé MeSH
- Publikační typ
- randomizované kontrolované studie MeSH
A validated, highly sensitive, and selective HPLC method with MS-MS detection has been developed for quantitative determination of azithromycin (AZI) in human Na2EDTA plasma. Roxithromycin (ROX) was used as internal standard. Human plasma containing AZI and internal standard was ultrafiltered through Centrifree Micropartition devices and the concentration of AZI was determined by isocratic HPLC-MS-MS. Multiple reaction monitoring mode (MRM) was used for MS-MS detection. The calibration plot was linear in the concentration range 2.55-551.43 ng mL(-1). Inter-day and Intra-day precision and accuracy of the proposed method were characterized by R.S.D and percentage deviation, respectively; both were less than 8%. Limit of quantification was 2.55 ng mL(-1). The proposed method was used to determine the pharmacokinetic profile of AZI (250-mg tablets).
- MeSH
- antibakteriální látky chemie krev MeSH
- azithromycin krev MeSH
- časové faktory MeSH
- EDTA krev MeSH
- hmotnostní spektrometrie metody MeSH
- lidé MeSH
- referenční hodnoty MeSH
- reprodukovatelnost výsledků MeSH
- roxithromycin chemie MeSH
- senzitivita a specificita MeSH
- ultrafiltrace MeSH
- vysokoúčinná kapalinová chromatografie metody MeSH
- Check Tag
- lidé MeSH
- Publikační typ
- validační studie MeSH
- MeSH
- adsorpce MeSH
- diuretika krev MeSH
- finanční podpora výzkumu jako téma MeSH
- fluorescenční spektrometrie MeSH
- furosemid krev MeSH
- kalibrace MeSH
- koncentrace vodíkových iontů MeSH
- referenční standardy MeSH
- reprodukovatelnost výsledků MeSH
- senzitivita a specificita MeSH
- vysokoúčinná kapalinová chromatografie metody MeSH
- MeSH
- finanční podpora výzkumu jako téma MeSH
- prazosin analýza chemie normy MeSH
- průtoková injekční analýza metody normy MeSH
- řízení kvality MeSH
- rozpustnost MeSH
- spektrofotometrie ultrafialová metody normy MeSH
- tablety MeSH
- techniky in vitro MeSH
- vysokoúčinná kapalinová chromatografie metody normy MeSH
